Weisbrod Samuel H, Marx Andreas
Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, 78457, Konstanz, Germany.
Chem Commun (Camb). 2008 Nov 30(44):5675-85. doi: 10.1039/b809528k. Epub 2008 Sep 25.
To broaden the scope of applications in DNA nano- and biotechnology, material science, diagnostics and molecular recognition the functionalization of DNA is of utmost importance. In the last decade many new methods have been developed to achieve this goal. Apart from the direct chemical synthesis of modified DNA by automated phosphoramidite chemistry incorporation of labelled triphosphates and the post-synthetic labelling approach evolved as valuable methods. New bioorthogonal reactions as Diels-Alder, click and Staudinger ligations pushed forward the post-synthetic approach as new insights into DNA polymerase substrate specificity allowed generation and amplification of labelled DNA strands. These novel developments are summarized herein.
为了拓宽DNA在纳米生物技术、材料科学、诊断学和分子识别等领域的应用范围，DNA的功能化至关重要。在过去十年中，已经开发出许多新方法来实现这一目标。除了通过自动亚磷酰胺化学直接化学合成修饰的DNA、掺入标记的三磷酸以及合成后标记方法外，还发展出了有价值的方法。新型生物正交反应如狄尔斯-阿尔德反应、点击反应和施陶丁格连接反应推动了合成后方法的发展，因为对DNA聚合酶底物特异性的新认识使得标记DNA链的生成和扩增成为可能。本文总结了这些新进展。
