利用新鲜分离的未成熟胚通过农杆菌介导法转化面包小麦和硬粒小麦。
Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos.
作者信息
Wu Huixia, Doherty Angela, Jones Huw D
机构信息
Genetic Resources and Enhancement Unit, CIMMYT, Mexico DF, Mexico.
出版信息
Methods Mol Biol. 2009;478:93-103. doi: 10.1007/978-1-59745-379-0_5.
PMID:19009440
Abstract
Agrobacterium-mediated transformation of wheat is becoming a viable alternative to the more established biolistic protocols. It offers advantages in terms of simple, low-copy-number integrations and can be applied with similar efficiencies to specific durum wheat and spring and winter bread wheat types varieties.
摘要
农杆菌介导的小麦转化正成为比更成熟的基因枪转化方法更可行的替代方案。它在简单的低拷贝数整合方面具有优势，并且可以以相似的效率应用于特定的硬粒小麦以及春小麦和冬小麦品种。
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