Hensel Goetz, Marthe Cornelia, Kumlehn Jochen
Plant Reproductive Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Seeland/OT Gatersleben, Germany.
Methods Mol Biol. 2017;1679:129-139. doi: 10.1007/978-1-4939-7337-8_8.
Methods of the Agrobacterium-mediated transformation of bread wheat (Triticum aestivum L.) have been improved in recent years so that genetic engineering can be routinely used for functional genomics as well as for wheat breeding. In the protocol described here, immature embryos of the spring-type model genotype Bobwhite SH 98 26 have been used. Preculture and temperature pretreatment of these explants have led to the reproducible generation of transgenic plants at efficiencies between 5 and 15%. Whereas primary transgenic plants regenerated in vitro commonly show reduced fitness and fertility, no apparent variations with regard to morphology and grain set in their transgenic progeny as compared to wild-type counterparts were observed.
近年来，农杆菌介导的面包小麦（Triticum aestivum L.）转化方法得到了改进，因此基因工程可常规用于功能基因组学以及小麦育种。在此描述的方案中，使用了春型模式基因型Bobwhite SH 98 26的未成熟胚。对这些外植体进行预培养和温度预处理后，可重复性地产生转基因植株，效率在5%至15%之间。虽然体外再生的初级转基因植株通常表现出适应性和育性降低，但与野生型对照相比，未观察到其转基因后代在形态和结实方面有明显差异。
