Harwood Wendy A, Smedley Mark A
Department of Crop Genetics, John Innes Centre, Colney, Norwich, UK.
Methods Mol Biol. 2009;478:125-36. doi: 10.1007/978-1-59745-379-0_8.
Microprojectile bombardment or biolistic techniques have been widely used for cereal transformation. These methods rely on the acceleration of gold particles, coated with plasmid DNA, into plant cells as a method of directly introducing the DNA. The first report of the generation of fertile, transgenic barley plants used biolistic techniques. However, more recently Agrobacterium-mediated transformation has been adopted as the method of choice for most cereals including barley. Biolistic procedures are still important for some barley transformation applications and also provide transient test systems for the rapid checking of constructs. This chapter describes methods for the transformation of barley using biolistic procedures and also highlights the use of the technology in transient assays.
微粒轰击法或生物弹道技术已被广泛用于谷类作物的转化。这些方法依靠将包被有质粒DNA的金颗粒加速打入植物细胞，以此作为直接导入DNA的一种方法。关于培育出可育转基因大麦植株的首次报道采用的是生物弹道技术。然而，最近农杆菌介导的转化法已成为包括大麦在内的大多数谷类作物的首选方法。生物弹道法对于某些大麦转化应用仍然很重要，并且还为快速检查构建体提供了瞬时测试系统。本章描述了使用生物弹道法转化大麦的方法，还强调了该技术在瞬时分析中的应用。
