[基因枪介导的春大麦（Hordeum vulgare L.）转化参数对绿色荧光蛋白报告基因瞬时表达水平的影响]

[The effect of the parameters of biolistic transformation of spring barley (Hordeum vulgare L.) on the level of transient expression of GFP reporter gene].

作者信息

Chernobrovkina M A, Sidorov E A, Baranov I A, Kharchenko P N, Dolgov S V

出版信息

Izv Akad Nauk Ser Biol. 2007 Nov-Dec(6):669-75.

PMID:19768960

Abstract

The effect of the parameters of biolistic transformation (rupture disk pressure of helium, vacuum pressure, stopping screen to target tissue distance, material (gold or tungsten) and size of particles, and duration of explant culturing before bombardment) on the level of transient expression of GFP reporter gene was studied in barley embryos. The highest transient expression was observed after explant preincubation for 12-14 days and bombardment with 1 microm gold particles at the helium pressure of 61.24-74.85 atm, vacuum pressure of 0.064 atm, and distance to target of 9 cm.

摘要

在大麦胚中研究了生物弹道转化参数（氦气破裂盘压力、真空压力、阻挡屏到靶组织的距离、材料（金或钨）及颗粒大小，以及轰击前外植体培养的持续时间）对绿色荧光蛋白（GFP）报告基因瞬时表达水平的影响。在外植体预培养12 - 14天，并用1微米金颗粒在氦气压力61.24 - 74.85个大气压、真空压力0.064个大气压及距靶标9厘米的距离下进行轰击后，观察到了最高的瞬时表达。