农杆菌介导的大麦（Hordeum vulgare L.）转化

Agrobacterium-mediated transformation of barley (Hordeum vulgare L.).

作者信息

Ismagul Ainur, Mazonka Iryna, Callegari Corinne, Eliby Serik

机构信息

Australian Centre for Plant Functional Genomics, University of Adelaide, PMB 1, Glen Osmond, SA, Australia.

出版信息

Methods Mol Biol. 2014;1145:203-11. doi: 10.1007/978-1-4939-0446-4_16.

DOI:10.1007/978-1-4939-0446-4_16

PMID:24816670

Abstract

Barley biotechnology requires efficient genetic engineering tools for producing transgenic plants necessary for conducting reverse genetics analyses in breeding and functional genomics research. Agrobacterium-mediated genetic transformation is an important technique for producing barley transgenics with simple low-copy number transgenes. This chapter reports a refined protocol for the systematic high-throughput transformation of the advanced Australian spring barley breeding line WI4330.

摘要

大麦生物技术需要高效的基因工程工具，以培育出用于育种反向遗传学分析和功能基因组学研究的转基因植物。农杆菌介导的遗传转化是一种用于培育具有简单低拷贝数转基因的大麦转基因植株的重要技术。本章报告了一种用于澳大利亚先进春大麦育种系WI4330系统高通量转化的优化方案。