Adelstein S, Pritchard-Briscoe H, Anderson T A, Crosbie J, Gammon G, Loblay R H, Basten A, Goodnow C C
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Science. 1991 Mar 8;251(4998):1223-5. doi: 10.1126/science.1900950.
Self-tolerance to a transgene-encoded protein, hen egg lysozyme, was examined in the T and B cell repertoires of a series of lines of transgenic mice that expressed different serum concentrations of soluble lysozyme. T cells were tolerant in all lines in which lysozyme was expressed irrespective of the antigen concentration, whereas B cell tolerance did not occur when the serum lysozyme concentration was less than 1.5 nanograms per milliliter (0.1 nM). Induction of elevated transgene expression could restore B cell tolerance. These findings support the hypothesis that autoimmune disease may in some instances arise through a bypass of T cell tolerance.
在一系列表达不同血清浓度可溶性溶菌酶的转基因小鼠品系的T细胞和B细胞库中，研究了对转基因编码蛋白——鸡卵溶菌酶的自身耐受性。无论抗原浓度如何，在所有表达溶菌酶的品系中T细胞均具有耐受性，而当血清溶菌酶浓度低于每毫升1.5纳克（0.1纳摩尔）时，B细胞耐受性并未出现。转基因表达升高的诱导可恢复B细胞耐受性。这些发现支持了自身免疫性疾病在某些情况下可能通过T细胞耐受性的旁路产生的假说。
