Lahiri Ramanuj, Sandoval Felipe G, Krahenbuhl James L, Shannon Edward J
Laboratory Research Branch, National Hansen's Disease Programs, Louisiana State University, Baton Rouge, 70803 USA.
Lepr Rev. 2008 Sep;79(3):311-4.
The immune-mediated events that precipitate erythema nodosum leprosum (ENL) are not well understood. One component may be the complexing of antibody with antigens released from infected macrophages, the activation of complement and the subsequent local inflammation. We assess here the ability of highly-purified, disrupted M. leprae, to activate complement.
Intact and sonically-disrupted crude and alkali-purified nu/nu mouse-derived M. leprae suspensions were incubated with normal serum and a hemolytic titer (CH50) was determined as a measure of complement fixation. Crude M. leprae consumed complement, and disrupted preparations more than the intact. Purified M. leprae preparations did not consume complement unless disrupted.
M. leprae, if disrupted, can activate complement. This supports a hypothesis that links released antigens with ENL, and may explain the increased probability of an occurrence of ENL following chemotherapy.
引发麻风结节性红斑（ENL）的免疫介导事件尚未完全明确。其中一个因素可能是抗体与受感染巨噬细胞释放的抗原形成复合物、补体激活以及随后的局部炎症。我们在此评估高度纯化的、经破碎处理的麻风杆菌激活补体的能力。
将完整的、经超声破碎处理的粗制及碱纯化的源自无胸腺裸鼠的麻风杆菌悬浮液与正常血清孵育，并测定溶血效价（CH50）作为补体固定的指标。粗制麻风杆菌消耗补体，且破碎处理后的制剂比完整的制剂消耗更多。纯化的麻风杆菌制剂除非经过破碎处理，否则不会消耗补体。
麻风杆菌若经破碎处理，能够激活补体。这支持了一种将释放的抗原与ENL联系起来的假说，并可能解释化疗后ENL发生概率增加的原因。
