Moscoso-Pérez Carmen, Moreda-Piñeiro Jorge, López-Mahía Purificación, Muniategui-Lorenzo Soledad, Fernández-Fernández Esther, Prada-Rodríguez Darío
Department of Analytical Chemistry, Faculty of Sciences, University of A Coruña, Campus da Zapateira s/n, E-15071, A Coruña, Spain.
J Chromatogr A. 2008 Dec 26;1215(1-2):15-20. doi: 10.1016/j.chroma.2008.10.100. Epub 2008 Nov 6.
This paper describes the development of a chelating solvent-based pressurized liquid extraction (PLE) method for the simultaneous extraction of As and Se species (As(III), As(V), Se(IV) and Se(VI)) in atmospheric particulate matter (PM(10)). The extracted As and Se species were evaluated by high performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS). The feasibility of several pressurized chelating solvents was recently investigated to leach As and Se species from atmospheric particulate matter. The best results (high recoveries) were obtained when using EDTA. Experimental design approaches were used to achieve the best compromise conditions for the simultaneous extraction of As and Se species by EDTA-PLE. Analytical performances, such as limits of detection (in the range of 0.01-0.02 ng m(-3)), quantification (in the range of 0.02-0.07 ng m(-3)), and repeatability of the over-all procedure ( approximately 10%) were established. Finally, As and Se species were determined in several atmospheric particulate matter (PM(10)) samples collected in an urban area of A Coruña city (northwest coast of Spain) in 2006. In all the atmospheric particulate matter samples that were analysed, As(V) and Se(IV) (in the range of 0.17-0.60 ng m(-3)) were the major species found.
本文描述了一种基于螯合溶剂的加压液体萃取（PLE）方法的开发，该方法用于同时萃取大气颗粒物（PM(10)）中的砷和硒物种（As(III)、As(V)、Se(IV)和Se(VI)）。萃取的砷和硒物种通过高效液相色谱-氢化物发生原子荧光光谱法（HPLC-HG-AFS）进行评估。最近研究了几种加压螯合溶剂从大气颗粒物中浸出砷和硒物种的可行性。使用乙二胺四乙酸（EDTA）时获得了最佳结果（高回收率）。采用实验设计方法来实现EDTA-PLE同时萃取砷和硒物种的最佳折衷条件。确定了分析性能，如检测限（范围为0.01 - 0.02 ng m(-3)）、定量限（范围为0.02 - 0.07 ng m(-3)）以及整个过程的重复性（约10%）。最后，对2006年在西班牙西北海岸拉科鲁尼亚市市区采集的几个大气颗粒物（PM(10)）样品中的砷和硒物种进行了测定。在所有分析的大气颗粒物样品中，As(V)和Se(IV)（范围为0.17 - 0.60 ng m(-3)）是主要检测到的物种。
