Adil Bilal, Xiang Quanju, He Maolan, Wu Yuetong, Asghar Muhammad Ahsan, Arshad Muhammad, Qin Peng, Gu Yunfu, Yu Xiumei, Zhao Ke, Zhang Xiaoping, Ma Menggen, Chen Qiang, Chen Xiaoqiong, Yan Yanhong
College of Resource, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People's Republic of China.
Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People's Republic of China.
AMB Express. 2020 Mar 14;10(1):47. doi: 10.1186/s13568-020-00985-w.
Lentinan is a Lentinus edodes secondary metabolite that can regulate human immune function, but yields are low. Here, the effects of Ca and Na on L. edodes lentinan content were investigated. Metal ion concentrations and induction times were optimized according to mycelial biomass, and intracellular polysaccharide (IPS), extracellular polysaccharide (EPS), and total polysaccharide (TPS) content. The activities and gene expression of phospho-glucose isomerase (PGI), phosphoglucomutase (PGM), and UDP-glcpyrophosphorylase (UGP) were also measured. Ca and Na concentration and induction time affected biomass, IPS, and EPS concentrations. Na increased EPS, IPS and TPS, while Ca increased biomass, IPS, and TPS. During fermentation, mycelial biomass varied greatly under Ca induction, while IPS, EPS and TPS varied greatly under Na induction. PGM and UGP activities increased in the presence of Na, while PGI increased with Ca. Compared to control samples, pgi and pgm expression under Na was greater at days 45 and 60, respectively, while under Ca, ugp expression was greater at day 45. IPS content correlated significantly with enzyme activity, while EPS correlated with PGM activity. Our data contributes to better understanding how Na and Ca affect mycelial growth and secondary metabolite production, and of polysaccharide biosynthesis mechanisms of L. edodes.
香菇多糖是香菇的一种次生代谢产物，能够调节人体免疫功能，但产量较低。在此，研究了钙和钠对香菇香菇多糖含量的影响。根据菌丝体生物量、细胞内多糖（IPS）、细胞外多糖（EPS）和总多糖（TPS）含量对金属离子浓度和诱导时间进行了优化。还测定了磷酸葡萄糖异构酶（PGI）、磷酸葡萄糖变位酶（PGM）和UDP-葡萄糖焦磷酸化酶（UGP）的活性及基因表达。钙和钠的浓度以及诱导时间影响生物量、IPS和EPS浓度。钠增加了EPS、IPS和TPS，而钙增加了生物量、IPS和TPS。在发酵过程中，钙诱导下菌丝体生物量变化很大，而钠诱导下IPS、EPS和TPS变化很大。在钠存在的情况下，PGM和UGP活性增加，而PGI活性随钙增加。与对照样品相比，在第45天和第60天，钠处理下pgi和pgm的表达分别更高，而在钙处理下，第45天ugp的表达更高。IPS含量与酶活性显著相关，而EPS与PGM活性相关。我们的数据有助于更好地理解钠和钙如何影响菌丝体生长和次生代谢产物的产生，以及香菇多糖的生物合成机制。
