Ansari F, Grigoriev P, Libor S, Tothill I E, Ramsden J J
Microsystems & Nanotechnology Centre, Cranfield University, Bedfordshire MK430AL, UK.
Biotechnol Bioeng. 2009 Apr 1;102(5):1505-12. doi: 10.1002/bit.22161.
Biodesulfurization (BDS) of dibenzothiophene (DBT) was carried out by Rhodococcus erythropolis IGST8 decorated with magnetic Fe3O4 nanoparticles, synthesized in-house by a chemical method, with an average size of 45-50 nm, in order to facilitate the post-reaction separation of the bacteria from the reaction mixture. Scanning electron microscopy (SEM) showed that the magnetic nanoparticles substantially coated the surfaces of the bacteria. It was found that the decorated cells had a 56% higher DBT desulfurization activity in basic salt medium (BSM) compared to the nondecorated cells. We propose that this is due to permeabilization of the bacterial membrane, facilitating the entry and exit of reactant and product, respectively. Model experiments with black lipid membranes (BLM) demonstrated that the nanoparticles indeed enhance membrane permeability.
用化学方法在内部合成的平均尺寸为45 - 50纳米的磁性Fe3O4纳米颗粒修饰的红平红球菌IGST8对二苯并噻吩（DBT）进行生物脱硫（BDS），以便于反应后从反应混合物中分离细菌。扫描电子显微镜（SEM）显示磁性纳米颗粒大量覆盖在细菌表面。结果发现，与未修饰的细胞相比，修饰后的细胞在碱性盐培养基（BSM）中对DBT的脱硫活性高56%。我们认为这是由于细菌细胞膜的通透性增加，分别促进了反应物和产物的进出。用黑色脂质膜（BLM）进行的模型实验表明，纳米颗粒确实增强了膜的通透性。
