猴疱疹病毒的部分纯化及生化特性分析
Partial purification and biochemical characterization of herpesvirus saimiri.
作者信息
Aaslestad H G, Twardzik D R
出版信息
IARC Sci Publ (1971). 1975(11 Pt 1):269-75.
PMID:190128
Abstract
HVS propagated in Vero cells was labelled with 3H-leucine and purified by isopycnic flotation in sucrose. Electron microscopic examination revealed both complete and incomplete HVS. No significant contamination with cellular debris was noted. Polyacrylamide gel electrophoresis analysis of solubilized virus revealed at least 12 proteins varying in molecular weight from 8 000 to 200 000 daltons.
摘要
在非洲绿猴肾细胞中繁殖的人疱疹病毒 6 型（HVS）用³H-亮氨酸标记，并通过在蔗糖中的等密度浮选进行纯化。电子显微镜检查发现了完整和不完整的 HVS。未发现明显的细胞碎片污染。对溶解的病毒进行的聚丙烯酰胺凝胶电泳分析显示至少有 12 种蛋白质，其分子量在 8000 至 200000 道尔顿之间变化。
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