Simonds J A, Robey W G, Graham B J, Oie H, Vande Woude G F
Arch Virol. 1975;49(2-3):249-59. doi: 10.1007/BF01317543.
Conditions for growth, concentration, and purification of Herpesvirus saimiri were determined. Optimal yields of infectious Herpesvirus saimiri (HVS) were obtained from infected owl monkey kidney (OMK) cells grown at 32.5 degrees C in medium containing 10 per cent fetal calf serum. Forth-five percent of the initial infectious HVS was recovered after an 18-fold concentration using 8 per cent polyethylene glycol 6000 in the presence of 0.5 M NaCl. Polyethylene glycol concentrated HVS was purified in an isopycnic-linear Renografin gradient (1.0-1.3 g/cm3. Ninety-six percent of the infectivity was recovered in a single 1.16 g/cm3 density region. DNA extracted from purified HVS was resolved into two distinct density classes by CsCl equilibrium centrifugation (1.727 and 1.709 g/cm3). DNase treated HVA virions yield four DNA species with densities of 1.727, 1.718, 1.712, and 1.706 g/cm3 in CsCl centrifugation.
确定了赛米里疱疹病毒的生长、浓缩和纯化条件。在含有10%胎牛血清的培养基中，于32.5摄氏度培养的受感染猫头鹰猴肾（OMK）细胞可获得传染性赛米里疱疹病毒（HVS）的最佳产量。在0.5M氯化钠存在的情况下，使用8%聚乙二醇6000进行18倍浓缩后，可回收初始传染性HVS的45%。聚乙二醇浓缩的HVS在等密度线性泛影葡胺梯度（1.0 - 1.3 g/cm³）中纯化。96%的感染力在单一的1.16 g/cm³密度区域中回收。通过氯化铯平衡离心法（1.727和1.709 g/cm³），从纯化的HVS中提取的DNA被解析为两个不同的密度类别。经脱氧核糖核酸酶处理的HVA病毒粒子在氯化铯离心时产生四种密度分别为1.727、1.718、1.712和1.706 g/cm³的DNA种类。
