Falk L, Wright J, Deinhardt F, Wolfe L, Schaffer P, Benyesh-Melnick M
Cancer Res. 1976 Feb;36(2 pt 2):707-10.
Herpesvirus saimiri (HVS) was propagated in vero cells for 3 passages at 39 degrees and cloned 3 times at 34 degrees. This virus was inoculated into cotton-topped marmoset and squirrel monkeys; all inoculated monkeys became infected as HVS was reisolated after their circulating lymphocytes were cultured with vero cells and measurable levels of antiviral antibodies developed that were measured by immunofluorescence and/or neutralization tests. None of the inoculated monkeys developed any signs of overt disease and all inoculated monkeys have survived 9 to 14 months postinoculation. The attenuated virus appears to be genetically stable as virus isolated from an infected marmoset was passed 3 times in vitro and then inoculated into other marmosets, which became infected and remained clinically well. Marmosets latently infected with attenuated HVS were not protected when challenged with a large dose (770 plaque-forming units) of oncogenic HVS, although these marmosets survived about 3 times longer than did inoculated control marmosets.
赛米利疱疹病毒（HVS）在非洲绿猴肾细胞中于39℃传代3次，并于34℃克隆3次。将该病毒接种到棉顶狨猴和松鼠猴体内；所有接种的猴子均被感染，因为在用非洲绿猴肾细胞培养其循环淋巴细胞后重新分离出了HVS，并且通过免疫荧光和/或中和试验检测到了可测量水平的抗病毒抗体。接种的猴子均未出现任何明显疾病迹象，所有接种的猴子在接种后9至14个月均存活。减毒病毒似乎在基因上是稳定的，因为从受感染的狨猴中分离出的病毒在体外传代3次，然后接种到其他狨猴体内，这些狨猴被感染但临床上仍健康。潜伏感染减毒HVS的狨猴在受到大剂量（770个空斑形成单位）致癌HVS攻击时未受到保护，尽管这些狨猴的存活时间比接种的对照狨猴长约3倍。
