Enríquez Rocío, Abad Raquel, Salcedo Celia, Vázquez Julio A
National Centre for Microbiology, National Institute of Health Carlos III, Majadahonda (Madrid), Spain.
Antimicrob Agents Chemother. 2009 Feb;53(2):796-7. doi: 10.1128/AAC.00531-08. Epub 2008 Nov 17.
Recently the CLSI recommended a disk diffusion method and breakpoints for meningococci which include breakpoints derived for nalidixic acid which serve as surrogate markers for gyrase A mutations associated with diminished fluoroquinolone susceptibility. This study presents the application of this methodology to a panel of 57 meningococcal strains isolated in Spain that include all levels of susceptibility to ciprofloxacin. In conclusion, the most useful method to predict isolates with gyrA mutations that decrease the activity of fluoroquinolones is the use of 30-microg nalidixic acid disks.
最近，临床和实验室标准协会（CLSI）推荐了一种针对脑膜炎球菌的纸片扩散法及折点，其中包括萘啶酸的折点，这些折点可作为与氟喹诺酮敏感性降低相关的gyrase A突变的替代标志物。本研究介绍了该方法在一组57株西班牙分离的脑膜炎球菌菌株中的应用，这些菌株对环丙沙星具有不同程度的敏感性。总之，预测具有降低氟喹诺酮活性的gyrA突变菌株的最有用方法是使用含30μg萘啶酸的纸片。
