基本模式分析：一种用于表征细胞代谢的有用代谢途径分析工具。
Elementary mode analysis: a useful metabolic pathway analysis tool for characterizing cellular metabolism.
作者信息
Trinh Cong T, Wlaschin Aaron, Srienc Friedrich
机构信息
Department of Chemical Engineering and Materials Science, University of Minnesota, 151 Amundson Hall, 421 Washington Ave SE, Minneapolis, MN 55455, USA.
出版信息
Appl Microbiol Biotechnol. 2009 Jan;81(5):813-26. doi: 10.1007/s00253-008-1770-1. Epub 2008 Nov 15.
Abstract
Elementary mode analysis is a useful metabolic pathway analysis tool to identify the structure of a metabolic network that links the cellular phenotype to the corresponding genotype. The analysis can decompose the intricate metabolic network comprised of highly interconnected reactions into uniquely organized pathways. These pathways consisting of a minimal set of enzymes that can support steady state operation of cellular metabolism represent independent cellular physiological states. Such pathway definition provides a rigorous basis to systematically characterize cellular phenotypes, metabolic network regulation, robustness, and fragility that facilitate understanding of cell physiology and implementation of metabolic engineering strategies. This mini-review aims to overview the development and application of elementary mode analysis as a metabolic pathway analysis tool in studying cell physiology and as a basis of metabolic engineering.
摘要
基本模式分析是一种有用的代谢途径分析工具，用于识别将细胞表型与相应基因型联系起来的代谢网络结构。该分析可以将由高度相互连接的反应组成的复杂代谢网络分解为独特组织的途径。这些由一组能够支持细胞代谢稳态运行的最小酶组成的途径代表了独立的细胞生理状态。这种途径定义为系统地表征细胞表型、代谢网络调节、稳健性和脆弱性提供了严格的基础，有助于理解细胞生理学和实施代谢工程策略。本综述旨在概述基本模式分析作为一种代谢途径分析工具在研究细胞生理学以及作为代谢工程基础方面的发展和应用。
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