Shawar R M, Salyer J L, Ballou D, Clarridge J E
Department of Pathology, Baylor College of Medicine, Veterans Administration Medical Center, Houston, Texas, USA.
Diagn Microbiol Infect Dis. 1995 Jan;21(1):15-9. doi: 10.1016/0732-8893(94)00112-a.
We report the recovery of Coccidioides immitis from the blood and abscess fluid of two separate patients by using two automated blood culture systems. In the first case, an aspirate from a neck abscess containing C. immitis spherules was serially diluted and inoculated into liquid media used by the BacT/Alert and the Bactec NR660 blood culture systems. BacT/Alert bottles inoculated with 10(5), 10(4), 10(3), 10(2), 10 and two spherules produced a positive signal at 19, 24, 35, 42, 57, and 62 h postinoculation, respectively. Bactec NR660 bottles containing > 10(2) shperules and 10 spherules produced a positive signal after approximately 72 and 96 h of incubation, respectively. In the second case, a blood specimen incubated in BacT/Alert blood culture both was signaled positive after 82 h of incubation. No organisms were detected by Gram stain of the broth, but C. immitis grew after blind subculture. Our observations demonstrate that these rapid blood culture systems are capable of supporting growth of C. immitis. To our knowledge, this report is the first to detect C. immitis by these blood culture systems.
我们报告了通过使用两种自动血培养系统，从两名不同患者的血液和脓肿液中分离出粗球孢子菌。在第一个病例中，将含有粗球孢子菌小球体的颈部脓肿抽吸物进行连续稀释，并接种到BacT/Alert和Bactec NR660血培养系统所使用的液体培养基中。接种了10(5)、10(4)、10(3)、10(2)、10和两个小球体的BacT/Alert瓶分别在接种后19、24、35、42、57和62小时产生阳性信号。含有>10(2)个小球体和10个小球体的Bactec NR660瓶分别在培养约72和96小时后产生阳性信号。在第二个病例中，在BacT/Alert血培养瓶中培养的一份血液标本在培养82小时后发出阳性信号。肉汤的革兰氏染色未检测到微生物，但盲传继代培养后粗球孢子菌生长。我们的观察表明，这些快速血培养系统能够支持粗球孢子菌的生长。据我们所知，本报告是首次通过这些血培养系统检测到粗球孢子菌。
