Rose M E, Wakelin D, Hesketh P
Houghton Laboratory, AFRC Institute for Animal Health, Huntingdon, Cambridgeshire, UK.
Parasite Immunol. 1991 Jan;13(1):63-74. doi: 10.1111/j.1365-3024.1991.tb00263.x.
The effect of treatment with a monoclonal antibody (MoAb) capable of neutralising interferon-gamma (IFN-gamma) on the course of coccidial infections in mice (C57BL/6 and NIH infected with Eimeria vermiformis or E. pragensis, and BALB/c infected with E. pragensis) was examined. The results differed with the species of parasite, the strain of mouse, the measure of infection and whether the infection was a primary or secondary one. The replication of E. vermiformis in primary infections was enhanced in both C57BL/6 and NIH mice, but less MoAb was required in NIH than in C57BL/6 to produce similar effects. In neither strain did treatment prevent priming or interfere with the complete immunity to challenge normally induced by moderate infection with E. vermiformis. The replication of E. pragensis in primary infections was not affected in any of the strains of mouse but the clinical effects were exacerbated. Priming with E. pragensis was unaffected by treatment but the partial immunity to challenge, normally induced by infection with this species, was reduced when MoAb was given 2 h before challenge. This reduction was evident as an increased faecal output of oocysts and loss of body weight. These results confirm the role of IFN-gamma in resistance to coccidiosis and further emphasise the complexity of the immune response in this disease.
研究了用一种能够中和干扰素 -γ（IFN -γ）的单克隆抗体（MoAb）治疗对小鼠球虫感染病程的影响（C57BL/6和NIH小鼠感染蠕虫艾美耳球虫或普拉格艾美耳球虫，BALB/c小鼠感染普拉格艾美耳球虫）。结果因寄生虫种类、小鼠品系、感染程度以及感染是原发性还是继发性而有所不同。在原发性感染中，蠕虫艾美耳球虫在C57BL/6和NIH小鼠中的复制均增强，但在NIH小鼠中产生类似效果所需的单克隆抗体比C57BL/6小鼠少。在这两种品系中，治疗均未阻止初次免疫或干扰由蠕虫艾美耳球虫中度感染正常诱导的对攻击的完全免疫。在原发性感染中，普拉格艾美耳球虫在任何小鼠品系中的复制均未受影响，但临床症状加重。用普拉格艾美耳球虫进行初次免疫不受治疗影响，但在攻击前2小时给予单克隆抗体时，该物种感染正常诱导的对攻击的部分免疫会降低。这种降低表现为卵囊粪便排出量增加和体重减轻。这些结果证实了IFN -γ在抗球虫病中的作用，并进一步强调了该疾病免疫反应的复杂性。
