Rose M E, Wakelin D, Hesketh P
Houghton Laboratory, Institute for Animal Health, Huntingdon, Cambridgeshire.
Infect Immun. 1989 May;57(5):1599-603. doi: 10.1128/iai.57.5.1599-1603.1989.
Neutralization of endogenous gamma interferon by treatment with a rat monoclonal antibody caused enhancement of infection with the protozoon Eimeria vermiformis in naive BALB/c mice. The effect was dose dependent and was apparent when a monoclonal antibody was given at 2 h before infection or up to 7 days postinfection, but it decreased with increasing time postinfection between days 4 and 7. The titers of parasite-specific antibodies in the serum were not significantly affected by the injection of monoclonal antibodies. Treatment during priming did not prevent the development of resistance to challenge, and treatment at the time of challenge did not abrogate established immunity. The results indicate that gamma interferon is involved in the control of primary infection with E. vermiformis in BALB/c mice but not in the expression of immunity to challenge.
用大鼠单克隆抗体处理以中和内源性γ干扰素，可导致未感染过的BALB/c小鼠感染蠕虫状艾美耳球虫的情况加剧。这种效应具有剂量依赖性，当在感染前2小时或感染后长达7天给予单克隆抗体时效应明显，但在感染后第4天至第7天之间，随着感染后时间的增加效应减弱。血清中寄生虫特异性抗体的滴度未受到单克隆抗体注射的显著影响。初次免疫期间进行处理并未阻止对攻击产生抗性的发展，而在攻击时进行处理也未消除已建立的免疫力。结果表明，γ干扰素参与了BALB/c小鼠对蠕虫状艾美耳球虫原发性感染的控制，但不参与对攻击免疫的表达。
