Tamkun J W, Kahn R A, Kissinger M, Brizuela B J, Rulka C, Scott M P, Kennison J A
Department of Biology, University of California, Santa Cruz 95064.
Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3120-4. doi: 10.1073/pnas.88.8.3120.
We have identified a Drosophila gene (arflike, arl) encoding a protein that is structurally related (approximately 55% identity) to the ADP-ribosylation factors (ARFs) of yeast and mammals. Biochemical analyses of purified recombinant arl-encoded protein revealed properties similar to the ARF proteins, including the ability to bind and hydrolyze GTP. Clear functional differences between arl and ARF proteins, including a complete lack of ARF activity, suggest that arl is not a functional homolog of ARF. A recessive lethal arl mutation was recovered, demonstrating that the arl locus is an essential gene. We conclude that the arl locus encodes an essential member of the ARF subfamily of small GTP-binding proteins in Drosophila.
我们已经鉴定出一个果蝇基因（arlike，arl），它编码一种蛋白质，该蛋白质在结构上与酵母和哺乳动物的ADP-核糖基化因子（ARFs）相关（约55%的同源性）。对纯化的重组arl编码蛋白的生化分析揭示了其与ARF蛋白相似的特性，包括结合和水解GTP的能力。arl和ARF蛋白之间明显的功能差异，包括完全缺乏ARF活性，表明arl不是ARF的功能同源物。我们获得了一个隐性致死的arl突变，这表明arl基因座是一个必需基因。我们得出结论，arl基因座编码果蝇中小GTP结合蛋白ARF亚家族的一个必需成员。
