Cupples Catherine M, Champagne Jarrod R, Lewis Kristen E, Cruz Tracey Dawson
Laboratory Corporation of America, Forensic Identity Department, 1912 Alexander Drive, Research Triangle Park, NC 27709, USA.
J Forensic Sci. 2009 Jan;54(1):103-7. doi: 10.1111/j.1556-4029.2008.00914.x. Epub 2008 Nov 10.
Screening methods capable of identifying DNA samples that will not yield short tandem repeat (STR) profiles are desired. In the past, quantitation methods have not been sensitive enough for this purpose. In this study, low level DNA samples were used to assess whether Quantifiler has a minimum quantitation value below which STR profiles would consistently fail to be detected. Buccal swabs were obtained and the DNA extracted, quantified, and serially diluted to concentrations ranging from 0.002 to 0.250 ng/microL. Samples were analyzed once with Quantifiler, followed by Profiler Plus amplification and capillary electrophoresis analysis. An absolute minimum value below which STR results were unobtainable could not be defined. From the 96 low level samples tested, STR loci (including one full profile) were successfully amplified and detected from 27% of the samples "undetected" by Quantifiler. However, no STR alleles were detected in 73% of these "undetected" samples, indicating that Quantifiler data may be useful for predicting STR typing success.
需要能够识别无法产生短串联重复序列（STR）图谱的DNA样本的筛选方法。过去，定量方法在这方面的灵敏度还不够。在本研究中，使用低水平DNA样本评估Quantifiler是否存在一个最低定量值，低于该值STR图谱将始终无法检测到。采集颊拭子，提取、定量DNA，并将其连续稀释至浓度范围为0.002至0.250 ng/μL。样本先用Quantifiler分析一次，然后进行Profiler Plus扩增和毛细管电泳分析。无法定义一个低于该值就无法获得STR结果的绝对最小值。在测试的96个低水平样本中，从Quantifiler“未检测到”的样本中有27%成功扩增并检测到了STR位点（包括一个完整图谱）。然而，在这些“未检测到”的样本中有73%未检测到STR等位基因，这表明Quantifiler数据可能有助于预测STR分型的成功。
