Hu L B, Zhang T, Yang Z M, Zhou W, Shi Z Q
School of Food, Henan Institute of Science and Technology, Xinxiang, China.
Lett Appl Microbiol. 2009 Jan;48(1):84-9. doi: 10.1111/j.1472-765X.2008.02493.x.
To understand the role of fengycins in regulating the fumonisin B1 (FB1)production of Fusarium verticillioides.
The mass ratio of FB1 to mycelia was determined in order to identify the effect of fengycins on FB1 production. It was shown that the amount of FB1 produced by unit mass mycelia decreased to 28% of the control. Results from mycelia resuspension with fengycins also demonstrated that fengycins had a potent impact on FB1 production. Gene expression patterns using quantitative reverse-transcription PCR (RT-PCR) revealed that the transcriptional levels of both FUM1 and FUM8 (coding enzymes for the generation of FB1) were down-regulated with fengycin treatment.
Fengycins could down-regulate the transcription of some key genes involved in the production of FB1, and impair FB1 synthesis by F. verticillioides.
These results further improved our understanding of fengycins as the potential candidates to control FB1 contamination in crops and food.
了解丰原素在调控轮枝镰孢菌伏马毒素B1（FB1）产生中的作用。
测定FB1与菌丝体的质量比，以确定丰原素对FB1产生的影响。结果表明，单位质量菌丝体产生的FB1量降至对照的28%。用丰原素重悬菌丝体的结果也表明，丰原素对FB1的产生有显著影响。使用定量逆转录PCR（RT-PCR）的基因表达模式显示，用丰原素处理后，FUM1和FUM8（编码产生FB1的酶）的转录水平均下调。
丰原素可下调参与FB1产生的一些关键基因的转录，并损害轮枝镰孢菌的FB1合成。
这些结果进一步加深了我们对丰原素作为控制作物和食品中FB1污染潜在候选物的理解。
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