Cawoy Hélène, Debois Delphine, Franzil Laurent, De Pauw Edwin, Thonart Philippe, Ongena Marc
Walloon Center for Industrial Microbiology, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium.
Microb Biotechnol. 2015 Mar;8(2):281-95. doi: 10.1111/1751-7915.12238. Epub 2014 Dec 19.
Some isolates of the Bacillus subtilis/amyloliquefaciens species are known for their plant protective activity against fungal phytopathogens. It is notably due to their genetic potential to form an impressive array of antibiotics including non-ribosomal lipopeptides (LPs). In the work presented here, we wanted to gain further insights into the relative role of these LPs in the global antifungal activity of B. subtilis/amyloliquefaciens. To that end, a comparative study was conducted involving multiple strains that were tested against four different phytopathogens. We combined various approaches to further exemplify that secretion of those LPs is a crucial trait in direct pathogen ward off and this can actually be generalized to all members of these species. Our data illustrate that for each LP family, the fungitoxic activity varies in function of the target species and that the production of iturins and fengycins is modulated by the presence of pathogens. Our data on the relative involvement of these LPs in the biocontrol activity and modulation of their production are discussed in the context of natural conditions in the rhizosphere.
枯草芽孢杆菌/解淀粉芽孢杆菌属的一些菌株以其对植物真菌病原体的保护活性而闻名。这主要归因于它们具有产生一系列令人印象深刻的抗生素的遗传潜力，包括非核糖体脂肽（LPs）。在本文所展示的研究中，我们希望进一步深入了解这些脂肽在枯草芽孢杆菌/解淀粉芽孢杆菌整体抗真菌活性中的相对作用。为此，我们进行了一项比较研究，涉及多个菌株，并针对四种不同的植物病原体进行了测试。我们结合了多种方法，进一步证明这些脂肽的分泌是直接抵御病原体的关键特性，并且这实际上可以推广到这些物种的所有成员。我们的数据表明，对于每个脂肽家族，其杀真菌活性因目标物种而异，并且伊枯草菌素和丰原素的产生受病原体存在的调节。我们将在根际自然条件的背景下讨论这些脂肽在生物防治活性中的相对作用及其产生的调节的数据。
