Key Laboratory of Integrated Management of Crop Diseases and Pests, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
Toxins (Basel). 2021 Nov 9;13(11):791. doi: 10.3390/toxins13110791.
and are fungal pathogens that cause diseases in cereal crops, such as Fusarium head blight (FHB), seedling blight, and stalk rot. They also produce a variety of mycotoxins that reduce crop yields and threaten human and animal health. Several strategies for controlling these diseases have been developed. However, due to a lack of resistant cultivars and the hazards of chemical fungicides, efforts are now focused on the biocontrol of plant diseases, which is a more sustainable and environmentally friendly approach. In the present study, the lipopeptide mycosubtilin purified from ATCC6633 significantly suppressed the growth of PH-1 and 7600 in vitro. Mycosubtilin caused the destruction and deformation of plasma membranes and cell walls in hyphae. Additionally, mycosubtilin inhibited conidial spore formation and germination of both fungi in a dose-dependent manner. experiments demonstrated the ability of mycosubtilin to control the adverse effects caused by and on wheat heads and maize kernels respectively. Mycosubtilin significantly decreased the production of deoxynivalenol (DON) and B-series fumonisins (FB, FB and FB) in infected grains, with inhibition rates of 48.92, 48.48, 52.42, and 59.44%, respectively. The qRT-PCR analysis showed that mycosubtilin significantly downregulated genes involved in mycotoxin biosynthesis. In conclusion, mycosubtilin produced by ATCC6633 was shown to have potential as a biological agent to control plant diseases and toxin contamination caused by and
和 是引起谷类作物病害的真菌病原体，如赤霉病（FHB）、幼苗疫病和茎腐病。它们还产生各种霉菌毒素，降低作物产量，威胁人类和动物健康。已经开发了几种控制这些疾病的策略。然而，由于缺乏抗性品种和化学杀菌剂的危害，目前的重点是植物病害的生物防治，这是一种更可持续和环保的方法。在本研究中，从 ATCC6633 中纯化的脂肽类抗真菌素 mycosubtilin 显著抑制了 PH-1 和 7600 在体外的生长。Mycosubtilin 导致菌丝中质膜和细胞壁的破坏和变形。此外，mycosubtilin 以剂量依赖的方式抑制两种真菌的分生孢子形成和萌发。田间试验表明，mycosubtilin 能够控制 和 对小麦穗和玉米穗分别造成的不良影响。Mycosubtilin 显著降低了受感染谷物中脱氧雪腐镰刀菌烯醇（DON）和 B 系列伏马菌素（FB、FB 和 FB）的产量，抑制率分别为 48.92%、48.48%、52.42%和 59.44%。qRT-PCR 分析表明，mycosubtilin 显著下调了与霉菌毒素生物合成相关的基因。总之， ATCC6633 产生的 mycosubtilin 具有作为生物制剂控制 和 引起的植物病害和毒素污染的潜力。
