Baba Hideo A, Wohlschlaeger Jeremias, Schmitz Klaus J, Nadalin Silvio, Lang Hauke, Benesch Alexandra, Gu Yanli, Biglarnia Ali-R, Sotiropoulos Georgios C, Takeda Atsushi, Takeda Nobuakira, von Wnuck Lipinski Karen, Levkau Bodo
Institute of Pathology and Neuropathology, University of Duisburg-Essen, Essen, Germany.
Liver Int. 2009 Apr;29(4):585-92. doi: 10.1111/j.1478-3231.2008.01911.x. Epub 2008 Oct 31.
Survivin regulates cell division and inhibits apoptosis. Liver regeneration is a complex process involving both proliferation and apoptosis. The role of survivin is not well elucidated and no data exist in humans.
Seventy per cent liver resection was used to investigate liver regeneration in rats. Survivin was identified by means of reverse transcriptase polymerase chain reaction, Western blotting and immunohistochemistry. Proliferation and apoptosis were quantified. Liver biopsies from 33 patients who underwent living donor liver transplantation were used to study survivin immuno-expression, proliferation and apoptosis within the first 17 days after transplantation. Seven healthy donors served as controls.
Survivin transcript and protein were significantly upregulated in rat hepatocytes after 24-72 h during regeneration and showed a significant correlation with proliferation but not with apoptosis. In humans, survivin was nearly absent in donor and reperfused liver tissue but increased significantly 5-7 days after transplantation and correlated with proliferation but not with apoptosis.
Survivin is upregulated in human and rodent liver regeneration and correlates with proliferation, suggesting an association of survivin and cell division.
生存素调节细胞分裂并抑制细胞凋亡。肝再生是一个涉及增殖和凋亡的复杂过程。生存素的作用尚未完全阐明，且在人类中尚无相关数据。
采用70%肝切除术研究大鼠肝再生。通过逆转录聚合酶链反应、蛋白质印迹法和免疫组织化学鉴定生存素。对增殖和凋亡进行定量分析。利用33例接受活体肝移植患者的肝活检组织研究移植后前17天内生存素的免疫表达、增殖和凋亡情况。7名健康供体作为对照。
在大鼠肝再生过程中，24 - 72小时后生存素转录本和蛋白在肝细胞中显著上调，且与增殖显著相关，但与凋亡无关。在人类中，供体肝组织和再灌注肝组织中几乎不存在生存素，但移植后5 - 7天显著增加，且与增殖相关，但与凋亡无关。
生存素在人类和啮齿动物肝再生中上调，并与增殖相关，提示生存素与细胞分裂有关。
