Xu C-L, Zhou J-B, Zhao B-T, Lan G-C, Luo M-J, Chang Z-L, Sui H-S, Tan J-H
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, Shandong, PR China.
Reprod Domest Anim. 2009 Oct;44(5):771-8. doi: 10.1111/j.1439-0531.2008.01071.x. Epub 2008 Nov 13.
The suitability of certain commercial and self-made chemically defined extenders for liquid storage of goat semen was tested and the effects of storage temperatures, dilution rates and sperm washing and pH of extenders on the goat sperm during liquid storage were observed. Semen was collected from nine goat bucks of the Lubei White and Boer breeds using an artificial vagina. Each ejaculate after initial evaluation was diluted with a specific extender, cooled and stored at a desired temperature. Stored semen was evaluated for sperm motility and other parameters every 24 or 48 h of storage. The ranking order of the existing milk- and yolk-free extenders in sustaining goat sperm motility was Androhep > Zorlesco > Beltsville thawing solution > the Tris-glucose medium. The new extender (mZA) which was formulated based on Zorlesco and Androhep was more suitable for goat sperm than Androhep. The mZAP extender with Bovine Serum Albumin (BSA) replaced with polyvinyl alcohol (PVA) worked as efficiently as the mZA in maintaining sperm motility, membrane integrity, acrosome intactness and capacitation status. Goat sperm motility was best maintained at 5 degrees C during liquid preservation, but decreased significantly as the temperature increased. When semen was sixfold diluted, sperm motility was maintained longer (p < 0.05) after centrifugation, but sperm motility did not differ between the centrifuged and non-centrifuged groups when semen was 11-fold diluted. When the extender pH was adjusted from 6.6 to 6.04, the efficiency increased significantly in both Androhep and mZAP. A forward sperm motility of 34% was maintained for 9 days when buck semen was 11-fold diluted and stored at 5 degrees C in mZAP, with pH adjusted to 6.04. It is concluded that for liquid storage of buck semen, the mZA extender was more suitable than other extenders; BSA can be replaced with PVA in mZA; centrifugation to remove seminal plasma can be omitted by adequate dilution; and the storage temperature and pH of extenders affected sperm motility significantly.
测试了某些市售和自制的化学成分明确的稀释液对山羊精液液态保存的适用性，并观察了保存温度、稀释比例、精子洗涤以及稀释液pH值对液态保存期间山羊精子的影响。使用人工阴道从9只鲁北白山羊和波尔山羊种公羊采集精液。每次射精经过初步评估后，用特定的稀释液进行稀释，冷却并保存在所需温度下。在保存的每24或48小时对保存的精液进行精子活力和其他参数评估。在维持山羊精子活力方面，现有的不含牛奶和蛋黄的稀释液的排名顺序为：Androhep＞Zorlesco＞贝尔茨维尔解冻液＞Tris-葡萄糖培养基。基于Zorlesco和Androhep配制的新型稀释液（mZA）比Androhep更适合山羊精子。用聚乙烯醇（PVA）替代牛血清白蛋白（BSA）的mZAP稀释液在维持精子活力、膜完整性、顶体完整性和获能状态方面与mZA效果相同。在液态保存期间，山羊精子活力在5℃时维持最佳，但随着温度升高显著下降。当精液进行6倍稀释时，离心后精子活力维持时间更长（p＜0.05），但当精液进行11倍稀释时，离心组和未离心组的精子活力没有差异。当稀释液pH值从6.6调整到6.04时，Androhep和mZAP的效果均显著提高。当公羊精液在pH值调整为6.04的mZAP中进行11倍稀释并保存在5℃时，精子前向运动活力可维持9天，达到34%。结论是，对于公羊精液的液态保存，mZA稀释液比其他稀释液更合适；mZA中可以用PVA替代BSA；通过适当稀释可以省略离心去除精浆的步骤；稀释液的保存温度和pH值对精子活力有显著影响。
