使用加压液体萃取法同时从小型组织样本中提取和净化多氯联苯及其代谢物。
Simultaneous extraction and clean-up of polychlorinated biphenyls and their metabolites from small tissue samples using pressurized liquid extraction.
作者信息
Kania-Korwel Izabela, Zhao Hongxia, Norstrom Karin, Li Xueshu, Hornbuckle Keri C, Lehmler Hans-Joachim
机构信息
Department of Occupational and Environmental Health, Collage of Public Health, University of Iowa, Iowa City, IA 52242, USA.
出版信息
J Chromatogr A. 2008 Dec 19;1214(1-2):37-46. doi: 10.1016/j.chroma.2008.10.089. Epub 2008 Oct 31.
Abstract
A pressurized liquid extraction-based method for the simultaneous extraction and in situ clean-up of polychlorinated biphenyls (PCBs), hydroxylated (OH)-PCBs and methylsulfonyl (MeSO(2))-PCBs from small (<0.5 g) tissue samples was developed and validated. Extraction of a laboratory reference material with hexane-dichloromethane-methanol (48:43:9, v/v) and Florisil as fat retainer allowed an efficient recovery of PCBs (78-112%; RSD: 13-37%), OH-PCBs (46+/-2%; RSD: 4%) and MeSO(2)-PCBs (89+/-21%; RSD: 24%). Comparable results were obtained with an established analysis method for PCBs, OH-PCBs and MeSO(2)-PCBs.
摘要
开发并验证了一种基于加压液体萃取的方法，用于从小（<0.5 g）组织样本中同时萃取和原位净化多氯联苯（PCBs）、羟基化（OH）-PCBs和甲基磺酰基（MeSO₂）-PCBs。用己烷-二氯甲烷-甲醇（48:43:9，v/v）和弗罗里硅土作为脂肪保留剂萃取实验室参考物质，可有效回收PCBs（78-112%；相对标准偏差：13-37%）、OH-PCBs（46±2%；相对标准偏差：4%）和MeSO₂-PCBs（89±21%；相对标准偏差：24%）。使用已建立的PCBs、OH-PCBs和MeSO₂-PCBs分析方法可获得类似结果。
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