Ahmad Maqbool, Hussain Ijaz, Khan Ahrar
Department of Theriogenology, University of Agriculture, Faisalabad, Pakistan.
Exp Toxicol Pathol. 2009 Jul;61(4):339-46. doi: 10.1016/j.etp.2008.10.002. Epub 2008 Nov 18.
This study was carried out on 30 male dwarf goats to determine the effects of cypermethrin (CY) on semen characteristics and at tissue level in testes. Animals were divided randomly into five equal groups and each group was dipped with 0%, 0.1%, 0.4%, 0.8% or 1.6% CY, on days 0 and 15. The semen was collected at day 0, then fortnightly till 75 days and evaluated for physical characteristics, sperm morphology and concentration. None of the parameters studied showed any statistical difference on days 0 and 15 of sample collection. From day 30, effect of CY on semen characteristics started to appear and was more pronounced from days 45 to 75. Significantly (P<0.01) decreased ejaculatory volume, motility percentage, mass activity, and concentration of spermatozoa were observed in treated groups as compared to control. With the treatment of CY, pH of semen became more alkaline. Semen color changed from creamy (control) to milky white to straw color in treated bucks. Percentage of abnormal spermatozoa (tailless, bent tails, coiled tailed) was much higher in treated animals than in control. Dead spermatozoa increased significantly (P<0.01) in the treatment groups from days 30 to 75 as compared to the control group. All these changes were dose dependent, being less with low CY dose and more even extensive with high CY dose. Slight to moderate improvement in the above parameters was observed at day 75 nearly in all treated groups. Grossly decreased weight of testis and cyanotic epididymides were observed in bucks treated with 1.6% CY. Histopathologically, degenerative changes and loss of spermatogonia, spermatocyte, Sertoli cells, spermatids, and spermatozoa in seminiferous tubules were also dose dependent. It was concluded that CY caused dose-dependent effects on all parameters studied. High doses of CY (0.8% and 1.6% solution) affected the parameters on semen characteristics, though this effect seems to be transient as improvement in these parameters was observed at day 75; however, CY in goats should be used with great care to avoid over-dosage.
本研究以30只雄性矮山羊为对象，旨在确定氯氰菊酯（CY）对精液特性以及睾丸组织水平的影响。将动物随机分为五组，每组数量相等，于第0天和第15天分别用0%、0.1%、0.4%、0.8%或1.6%的CY进行药浴。在第0天采集精液，之后每两周采集一次，直至第75天，并对精液的物理特性、精子形态和浓度进行评估。在样本采集的第0天和第15天，所研究的参数均未显示出任何统计学差异。从第30天起，CY对精液特性的影响开始显现，在第45天至第75天更为明显。与对照组相比，处理组的射精量、活力百分比、总体活动度和精子浓度显著（P<0.01）降低。经CY处理后，精液的pH值变得更偏碱性。处理后的公羊精液颜色从奶油色（对照组）变为乳白色再变为稻草色。处理组中异常精子（无尾、弯尾、卷尾）的百分比远高于对照组。与对照组相比，处理组在第30天至第75天死精子显著增加（P<0.01）。所有这些变化均呈剂量依赖性，低剂量CY时变化较小，高剂量CY时变化更明显甚至更广泛。几乎所有处理组在第75天时上述参数均有轻微至中度改善。用1.6%CY处理的公羊睾丸重量明显减轻，附睾发绀。组织病理学检查显示，曲细精管中的精原细胞、精母细胞、支持细胞、精子细胞和精子的退行性变化也呈剂量依赖性。研究得出结论，CY对所有研究参数均产生剂量依赖性影响。高剂量的CY（0.8%和1.6%溶液）影响精液特性参数，不过这种影响似乎是暂时的，因为在第75天观察到这些参数有所改善；然而，在山羊中使用CY时应格外小心，避免过量使用。
