Yousef M I, El-Demerdash F M, Al-Salhen K S
Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
J Environ Sci Health B. 2003 Jul;38(4):463-78. doi: 10.1081/PFC-120021666.
Cypermethrin is considered as one of the endocrine disruptors. Isoflavones play an important role in various physiological processes in the body. It has both estrogenic and antioxidant effects. The objective of this study was to evaluate the protective role of isoflavones (2 mg/kg B.W) on semen quality and plasma testosterone levels of male New Zealand White rabbits given sublethal dose (24 mg/kg BW every other day for 12 weeks) of cypermethrin. Results showed that treatment with cypermethrin caused a significant decreases (P < 0.05) in ejaculate volume, sperm concentration, total sperm output, sperm motility (%), total motile sperm per ejaculate (TMS), packed sperm volume (PSV), semen initial fructose and plasma testosterone. In addition, live body weight (LBW), dry matter intake (DMI) and relative weights of testes and epididymis were decreased. On the other hand, treatment with cypermethrin increased (P < 0.05) the numbers of abnormal and dead sperms, and initial hydrogen ion concentration (pH). Results indicated that the presence of isoflavones together with cypermethrin was capable to minimize its harmful effects. Treatment with isoflavones alone had positive effects on some semen characteristics in spite of it is considered as estrogen-like compound. Since it causes significant increases in libido (by decreasing the reaction time), PSV, sperm motility and TMS, while abnormal and dead sperm were reduced compared to control animals. Meanwhile, isoflavones had no negative effect on ejaculate volume, total sperm output, sperm concentration, initial fructose concentration, pH and plasma testosterone levels. Results demonstrated the beneficial influences of isoflavones in reducing the negative effects of cypermethrin on reproductive characteristics of mature male rabbits. Interestingly, data showed that isoflavones alone caused an improvement in some semen quality and had no negative effects on male fertility, and did not have negative effects on male fertility.
氯氰菊酯被认为是一种内分泌干扰物。异黄酮在身体的各种生理过程中发挥着重要作用。它具有雌激素和抗氧化作用。本研究的目的是评估异黄酮（2毫克/千克体重）对给予亚致死剂量（每隔一天24毫克/千克体重，持续12周）氯氰菊酯的雄性新西兰白兔精液质量和血浆睾酮水平的保护作用。结果表明，氯氰菊酯处理导致射精量、精子浓度、总精子输出量、精子活力（%）、每次射精的总活动精子数（TMS）、精液浓缩体积（PSV）、精液初始果糖和血浆睾酮显著降低（P<0.05）。此外，活体体重（LBW）、干物质摄入量（DMI）以及睾丸和附睾的相对重量均降低。另一方面，氯氰菊酯处理增加了（P<0.05）异常和死亡精子的数量以及初始氢离子浓度（pH）。结果表明，异黄酮与氯氰菊酯同时存在能够将其有害影响降至最低。尽管异黄酮被认为是一种类雌激素化合物，但单独使用异黄酮对某些精液特征有积极影响。因为它能显著提高性欲（通过缩短反应时间）、PSV、精子活力和TMS，同时与对照动物相比，异常和死亡精子减少。同时，异黄酮对射精量、总精子输出量、精子浓度、初始果糖浓度、pH和血浆睾酮水平没有负面影响。结果证明了异黄酮在减轻氯氰菊酯对成熟雄性兔生殖特征的负面影响方面的有益作用。有趣的是，数据表明单独使用异黄酮可改善某些精液质量，对雄性生育能力没有负面影响，并且对雄性生育能力没有负面影响。
