Lü Bingjian, Fang Yihu, Xu Jing, Wang Lipei, Xu Fangying, Xu Enping, Huang Qiong, Lai Maode
Department of Pathology, School of Medicine, Zhejiang University Department of Anatomical & Cellular Pathology, the Affiliated Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Am J Clin Pathol. 2008 Dec;130(6):897-904. doi: 10.1309/AJCPW1W8GJBQGCNI.
Our purpose was to investigate the role of SOX9, a novel downstream molecule of beta-catenin, in colorectal cancer. Expression of SOX9 and beta-catenin was detected by immunostaining, quantitative real-time reverse transcription-polymerase chain reaction (Q-PCR), and Western blot in colorectal cancer. The correlation between SOX9 or beta-catenin expression and clinicopathologic parameters was also analyzed. Immunostaining, Q-PCR, and Western blot consistently confirmed SOX9 up-regulation in colorectal cancer compared with normal mucosa (P < .05). Immunostaining showed more SOX9+ cells in the lower zone of colonic crypts than in the upper zone (P < .05). Cancers with strong SOX9 immunostaining were significantly associated with a lower 5-year overall survival (40% [17/43] vs low expression, 69% [66/95]; P < .01). The Cox proportional hazards model showed that strong SOX9 expression was an independent adverse prognosticator in colorectal cancer (P < .05). The detection of SOX9 expression might contribute to predicting clinical outcomes for patients with colorectal cancer.
我们的目的是研究β-连环蛋白的一种新型下游分子SOX9在结直肠癌中的作用。通过免疫染色、定量实时逆转录聚合酶链反应（Q-PCR）和蛋白质免疫印迹法检测结直肠癌中SOX9和β-连环蛋白的表达。还分析了SOX9或β-连环蛋白表达与临床病理参数之间的相关性。免疫染色、Q-PCR和蛋白质免疫印迹法一致证实，与正常黏膜相比，结直肠癌中SOX9表达上调（P < 0.05）。免疫染色显示，结肠隐窝下部区域的SOX9+细胞比上部区域更多（P < 0.05）。SOX9免疫染色强的癌症与较低的5年总生存率显著相关（40% [17/43] 对比低表达，69% [66/95]；P < 0.01）。Cox比例风险模型显示，SOX9强表达是结直肠癌的独立不良预后因素（P < 0.05）。检测SOX9表达可能有助于预测结直肠癌患者的临床结局。
