强直性脊柱炎中杀伤细胞免疫球蛋白样受体基因的分析
Analysis of killer immunoglobulin-like receptor genes in ankylosing spondylitis.
作者信息
Harvey D, Pointon J J, Sleator C, Meenagh A, Farrar C, Sun J Y, Senitzer D, Middleton D, Brown M A, Wordsworth B P
机构信息
Institute of Musculoskeletal Sciences, Botnar Research Centre, University of Oxford, Oxford, UK.
出版信息
Ann Rheum Dis. 2009 Apr;68(4):595-8. doi: 10.1136/ard.2008.095927. Epub 2008 Nov 19.
OBJECTIVES
To assess the possible association of killer immunoglobulin-like receptor (KIR) genes, specifically KIR3DL1, KIR3DS1 and KIR3DL2, with ankylosing spondylitis (AS).
METHODS
14 KIR genes were genotyped in 200 UK patients with AS and 405 healthy controls using multiplex polymerase chain reaction. Sequence-specific oligonucleotide probes were used to subtype 368 cases with AS and 366 controls for 12 KIR3DL2 alleles. Differences in KIR genotypes and KIR3DL2 allele frequencies were assessed using the chi(2) test.
RESULTS
KIR3DL1 and KIR3DS1 gene frequencies were very similar in cases with AS and controls (odds ratio = 1.5, 95% confidence interval 0.8 to 3.0, and odds ratio = 1.02, 95% confidence interval 0.2 to 5.3, respectively). KIR3DL2 allele frequencies were not significantly different between cases with AS and controls.
CONCLUSIONS
Neither the KIR gene content of particular KIR haplotypes nor KIR3DL2 polymorphisms contribute to AS.
目的
评估杀伤细胞免疫球蛋白样受体（KIR）基因，特别是KIR3DL1、KIR3DS1和KIR3DL2与强直性脊柱炎（AS）之间可能存在的关联。
方法
采用多重聚合酶链反应对200例英国AS患者和405名健康对照者的14个KIR基因进行基因分型。使用序列特异性寡核苷酸探针，对368例AS患者和366名对照者的12个KIR3DL2等位基因进行亚型分析。采用卡方检验评估KIR基因型和KIR3DL2等位基因频率的差异。
结果
AS患者和对照者中KIR3DL1和KIR3DS1基因频率非常相似（优势比分别为1.5，95%置信区间0.8至3.0；优势比为1.02，95%置信区间0.2至5.3）。AS患者和对照者之间KIR3DL2等位基因频率无显著差异。
结论
特定KIR单倍型的KIR基因组成以及KIR3DL2多态性均与AS无关。
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