Guo Han-Wen, Chen Chien-Tsun, Wei Yau-Huei, Lee Oscar K, Gukassyan Vladimir, Kao Fu-Jen, Wang Hsing-Wen
Institute of Biophotonics, National Yang-Ming University, 155 Li-Nong Street, Section 2, Taipei 11221, Taiwan.
J Biomed Opt. 2008 Sep-Oct;13(5):050505. doi: 10.1117/1.2990752.
The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.
通过还原型烟酰胺腺嘌呤二核苷酸（NADH）荧光寿命来研究人间充质干细胞（hMSCs）在成骨分化过程中的代谢变化。平均荧光寿命增加以及游离NADH与蛋白质结合的NADH之间的比例降低，这与我们之前报道的hMSCs在分化过程中三磷酸腺苷（ATP）水平升高相关。这些发现表明，NADH荧光寿命可能作为一种新的光学生物标志物，用于从分化后代中无创筛选干细胞。
