Kunapareddy Nagapratima, Freyer James P, Mourant Judith R
Los Alamos National Laboratory, Bioscience Division, MS E535, Los Alamos, New Mexico 87545, USA.
J Biomed Opt. 2008 Sep-Oct;13(5):054002. doi: 10.1117/1.2978061.
Raman spectroscopy has been used to estimate the biochemical changes due to necrosis in an in vitro model system comprised of a human malignant melanoma cell line (MEL-28). Combined oxygen and glucose deprivation was used to simulate necrotic cell death in tumors. Raman spectroscopy measurements of nonproliferating live cells and dead cells were made at 24, 48, and 72 hours. Quantitative estimates of the biochemical composition of live and dead cells were made by fitting cell spectra to the basis spectra of protein, lipid, RNA, DNA, and glycogen. A decrease in the relative amount of lipid and RNA, and an increase in the relative protein content, were observed in dead cells. A comparison of the spectra indicated the existence of conformational changes in protein and nucleic acids in dead cells. These results suggest that Raman spectroscopy could be used to detect necrotic cell death in tumors.
拉曼光谱已被用于评估在由人类恶性黑色素瘤细胞系（MEL - 28）组成的体外模型系统中由于坏死引起的生化变化。联合缺氧和葡萄糖剥夺被用于模拟肿瘤中的坏死性细胞死亡。在24、48和72小时对非增殖活细胞和死细胞进行了拉曼光谱测量。通过将细胞光谱拟合到蛋白质、脂质、RNA、DNA和糖原的基础光谱上，对活细胞和死细胞的生化组成进行了定量估计。在死细胞中观察到脂质和RNA相对含量的减少以及相对蛋白质含量的增加。光谱比较表明死细胞中蛋白质和核酸存在构象变化。这些结果表明拉曼光谱可用于检测肿瘤中的坏死性细胞死亡。
