Caspers P J, Lucassen G W, Wolthuis R, Bruining H A, Puppels G J
Department of General Surgery, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The Netherlands.
Biospectroscopy. 1998;4(5 Suppl):S31-9. doi: 10.1002/(SICI)1520-6343(1998)4:5+<S31::AID-BSPY4>3.0.CO;2-M.
Noninvasive techniques that provide detailed information about molecular composition, structure, and interactions are crucial to further our understanding of the relation between skin disease and biochemical changes in the skin, as well as for the development of penetration enhancers for transdermal drug administration. In this study we present in vitro and in vivo Raman spectra of human skin. Using a Raman microspectrometer, in vitro spectra were obtained of thin cross sections of human skin. They provided insight into the molecular composition of different skin layers. Evidence was found for the existence of a large variation in lipid content of the stratum corneum. A simple experimental setup for in vivo confocal Raman microspectroscopy of the skin was developed. In vivo Raman spectra of the stratum corneum were obtained at different positions of the arm and hand of three volunteers. They provided evidence for differences in the concentration of natural moisturizing factor at these positions.
能够提供有关分子组成、结构及相互作用详细信息的非侵入性技术，对于加深我们对皮肤疾病与皮肤生化变化之间关系的理解，以及开发用于透皮给药的渗透促进剂而言至关重要。在本研究中，我们展示了人体皮肤的体外和体内拉曼光谱。使用拉曼显微光谱仪，获取了人体皮肤薄切片的体外光谱。这些光谱让我们深入了解了不同皮肤层的分子组成。有证据表明角质层脂质含量存在很大差异。我们开发了一种用于皮肤体内共聚焦拉曼显微光谱分析的简单实验装置。在三名志愿者手臂和手部的不同位置获取了角质层的体内拉曼光谱。这些光谱证明了这些位置天然保湿因子浓度存在差异。
