Marino Vanessa, Galati Clelia, Arnone Claudio
University of Palermo, Dipartimento di Ingegneria Elettrica, Elettronica e delle Telecomunicazioni, Viale delle Scienze, edif. 9, Palermo, I-90128, Italy.
J Biomed Opt. 2008 Sep-Oct;13(5):054060. doi: 10.1117/1.2992142.
An optical technique for the enhancement of fluorescence detection sensitivity on planar samples is presented. Such a technique is based on the simultaneous optimization of excitation and light collection by properly combining interference and reflectance from the sample holder. Comparative tests have been performed in microarray applications, by evaluating the proposed solution against commercial glass-based devices, using popular labeling dyes, such as Cy3 and Cy5. The proposed technique is implemented on a substrate built with standard silicon technology and is therefore well suited for integrated micro total analysis systems (microTAS) applications.
本文提出了一种用于提高平面样品荧光检测灵敏度的光学技术。这种技术基于通过适当结合样品架的干涉和反射来同时优化激发和光收集。通过使用诸如Cy3和Cy5等流行的标记染料，针对商用玻璃基设备评估所提出的解决方案，已在微阵列应用中进行了对比测试。所提出的技术是在采用标准硅技术构建的基板上实现的，因此非常适合集成微全分析系统（microTAS）应用。
