Alanentalo Tomas, Lorén Christina E, Larefalk Asa, Sharpe James, Holmberg Dan, Ahlgren Ulf
Umeå University, Umeå Centre for Molecular Medicine, S-901 87, Umeå, Sweden.
J Biomed Opt. 2008 Sep-Oct;13(5):054070. doi: 10.1117/1.3000430.
A predicament when assessing the mechanisms underlying the pathogenesis of type-1 diabetes (T1D) has been to maintain simultaneous global and regional information on the loss of insulin-cell mass and the progression of insulitis. We present a procedure for high-resolution 3-D analyses of regions of interest (ROIs), defined on the basis of global assessments of the 3-D distribution, size, and shape of molecularly labeled structures within the full volume of the intact mouse pancreas. We apply a refined protocol for optical projection tomography (OPT)-aided whole pancreas imaging in combination with confocal laser scanning microscopy of site-directed pancreatic microbiopsies. As such, the methodology provides a useful tool for detailed cellular and molecular assessments of the autoimmune insulitis in T1D. It is anticipated that the same approach could be applied to other areas of research where 3-D molecular distributions of both global and regional character is required.
在评估1型糖尿病（T1D）发病机制背后的机制时，一个困境在于要同时保留有关胰岛细胞团丢失和胰岛炎进展的全局和局部信息。我们提出了一种对感兴趣区域（ROI）进行高分辨率三维分析的方法，该区域是根据对完整小鼠胰腺整个体积内分子标记结构的三维分布、大小和形状的全局评估来定义的。我们应用了一种改进的光学投影断层扫描（OPT）辅助全胰腺成像方案，并结合对定点胰腺活检组织进行共聚焦激光扫描显微镜检查。因此，该方法为T1D自身免疫性胰岛炎的详细细胞和分子评估提供了一个有用的工具。预计相同的方法可应用于其他需要全局和局部特征的三维分子分布的研究领域。
