Mazza G, Thorne K J, Richardson B A, Butterworth A E
Department of Pathology, University of Cambridge.
Eur J Immunol. 1991 Apr;21(4):901-5. doi: 10.1002/eji.1830210407.
IgG antibodies and eosinophils kill schistosomula of Schistosoma mansoni in vitro, and there is now evidence to suggest that the main factor that contributes to the expression of purified IgG effector function is the degree of activation of the donor's eosinophils. This study was designed to identify serum-derived activating factors in sera from individuals infected with S. mansoni. Such activating factors may be responsible for enhancing eosinophil cytotoxicity against schistosomula. Serum-borne mediators were prepared by fractionation of sera from infected individuals by gel filtration high-performance liquid chromatography. The eosinophil-stimulating activity of these mediators was assayed by a new method which depends on the increased expression of the CR3 alpha chain (CD11b) on the surface of activated eosinophils. Sera from infected individuals exhibited different levels of eosinophil activation, and activation appeared to be due to several serum factors, including interleukin 5. In conclusion, our results suggest that eosinophil-activating factors present in infection sera may not only be responsible for enhancing eosinophil cytotoxicity but also be necessary for its expression.
IgG抗体和嗜酸性粒细胞可在体外杀死曼氏血吸虫的童虫，目前有证据表明，促成纯化IgG效应子功能表达的主要因素是供体嗜酸性粒细胞的激活程度。本研究旨在鉴定曼氏血吸虫感染个体血清中的血清源性激活因子。此类激活因子可能负责增强嗜酸性粒细胞对童虫的细胞毒性。通过凝胶过滤高效液相色谱法对感染个体的血清进行分级分离，制备血清源性介质。采用一种新方法检测这些介质的嗜酸性粒细胞刺激活性，该方法依赖于活化嗜酸性粒细胞表面CR3α链（CD11b）表达的增加。感染个体的血清表现出不同水平的嗜酸性粒细胞激活，激活似乎归因于多种血清因子，包括白细胞介素5。总之，我们的结果表明，感染血清中存在的嗜酸性粒细胞激活因子可能不仅负责增强嗜酸性粒细胞的细胞毒性，而且对其表达也是必需的。
