Thorne K J, Richardson B A, Taverne J, Williamson D J, Vadas M A, Butterworth A E
Eur J Immunol. 1986 Sep;16(9):1143-9. doi: 10.1002/eji.1830160919.
Monocytes from moderately eosinophilic individuals secrete material that enhances the cytotoxic activity of eosinophils against antibody-coated schistosomula of Schistosoma mansoni. This material is not a single substance, but can be fractionated into several active components of different size and different charge. Gel filtration of mononuclear cell supernatants separated the eosinophil-activating activity into a major component of molecular mass of 40 kDa and a minor component of molecular mass of less than 10 kDa. The major component exhibited further heterogeneity on fractionation by high performance liquid chromatography. The bulk of the eosinophil-activating activity could be separated from both colony-stimulating factor (CSF) alpha activity and from tumor necrosis factor (TNF) activity. However, human recombinant CSF alpha (GM-CSF), human recombinant TNF and rabbit tumor necrosis serum all had eosinophil-activating activity when tested against schistosomula. Eosinophils were not activated by interleukin 1, interleukin 2, interferon-alpha, lipopolysaccharide or phorbol myristate acetate.
来自嗜酸性粒细胞适度增多个体的单核细胞分泌的物质可增强嗜酸性粒细胞对曼氏血吸虫抗体包被的童虫的细胞毒性活性。这种物质不是单一物质，而是可以分离成几种大小和电荷不同的活性成分。对单核细胞上清液进行凝胶过滤，将嗜酸性粒细胞激活活性分离为分子量40 kDa的主要成分和分子量小于10 kDa的次要成分。主要成分在高效液相色谱分离时表现出进一步的异质性。大部分嗜酸性粒细胞激活活性可与集落刺激因子（CSF）α活性和肿瘤坏死因子（TNF）活性分离。然而，人重组CSFα（GM-CSF）、人重组TNF和兔肿瘤坏死血清在针对童虫进行测试时均具有嗜酸性粒细胞激活活性。嗜酸性粒细胞不会被白细胞介素1、白细胞介素2、干扰素-α、脂多糖或佛波醇肉豆蔻酸酯激活。
