Cherrington C A, Hinton M, Pearson G R, Chopra I
Department of Veterinary Medicine, University of Bristol, Langford, Avon, UK.
J Appl Bacteriol. 1991 Feb;70(2):161-5. doi: 10.1111/j.1365-2672.1991.tb04442.x.
When strains of Escherichia coli K12 and Salmonella spp. were incubated with 0.5-0.7 mol/l formic or propionic acid at pH 5.0, propionic acid was more active than formic acid. It killed 90% of the cell population within 60 min compared with over 3 h for formic acid. Cell death was not associated with a reduction in culture turbidity or a loss of membrane integrity since morphologically normal membranes were observed by electron microscopy and only a small proportion of the cytoplasmic enzyme beta-galactosidase leaked into the supernatant fluid of acid-treated E. coli K12 cultures.
当大肠杆菌K12菌株和沙门氏菌属与0.5 - 0.7摩尔/升的甲酸或丙酸在pH 5.0条件下孵育时，丙酸比甲酸更具活性。它在60分钟内杀死了90%的细胞群体，而甲酸则需要超过3小时。细胞死亡与培养物浊度的降低或膜完整性的丧失无关，因为通过电子显微镜观察到形态正常的膜，并且只有一小部分细胞质酶β-半乳糖苷酶泄漏到经酸处理的大肠杆菌K12培养物的上清液中。
