Almendra Cláudia, Silva Teresa Luísa, Beja-Pereira Albano, Ferreira Ana Cristina, Ferrão-Beck Lénia, de Sá Maria Inácia Corrêa, Bricker Betsy J, Luikart Gordon
CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal.
Infect Genet Evol. 2009 Jan;9(1):104-7. doi: 10.1016/j.meegid.2008.10.007. Epub 2008 Oct 30.
We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus population genetic data to help discriminate among microbial isolates such as Brucella.
我们证明，“蹄印”检测法在区分小空间尺度（葡萄牙境内）收集的布鲁氏菌分离株方面具有很高的效能。此外，我们还说明了利用标记间的非随机关联进行单倍型鉴定如何能够区分马尔他布鲁氏菌生物变种（1型和3型）。我们建议，未来的研究在分析多位点群体遗传数据时使用单倍型鉴定，以帮助区分布鲁氏菌等微生物分离株。
