Chandwani S, Greco M A, Mittal K, Antoine C, Krasinski K, Borkowsky W
Department of Pediatrics, New York University Medical Center, New York.
J Infect Dis. 1991 May;163(5):1134-8. doi: 10.1093/infdis/163.5.1134.
The pathology of term placentas from seropositive human immunodeficiency virus (HIV)-infected and seronegative women was investigated by routine histologic, immunocytochemical, and in situ hybridization techniques. Placentas were evaluated for evidence of villitis, chorioamnionitis, and funisitis. Membranes, trophoblast, and decidua were also examined by immunohistochemistry using monoclonal HIV p24 antibody. Twenty placentas were evaluated by combined immunochemical and in situ hybridization techniques, using a 35S-labeled RNA probe complementary to the 3' long terminal repeat and envelope region of HIV-1. HIV-seropositive placentas did not show significant villitis; however, the incidence of chorioamnionitis increased (P less than .01). HIV antigens and nucleic acids were identified in the trophoblast of 10% of the placentas that also showed chorionitis. Term HIV-positive placentas may show histologic changes that may or may not be directly related to the virus. Analysis of tissues from earlier gestational placentas may prove more informative in clarifying the mechanism of maternal-fetal HIV transmission.
采用常规组织学、免疫细胞化学和原位杂交技术，对感染人类免疫缺陷病毒（HIV）血清阳性和血清阴性孕妇足月胎盘的病理学进行了研究。评估胎盘是否有绒毛炎、绒毛膜羊膜炎和脐带炎的迹象。还使用单克隆HIV p24抗体通过免疫组织化学检查胎膜、滋养层和蜕膜。采用与HIV-1的3'长末端重复序列和包膜区域互补的35S标记RNA探针，通过免疫化学和原位杂交联合技术对20个胎盘进行了评估。HIV血清阳性胎盘未显示明显的绒毛炎；然而，绒毛膜羊膜炎的发生率增加（P<0.01）。在10%同时显示绒毛膜炎的胎盘中，滋养层中鉴定出HIV抗原和核酸。足月HIV阳性胎盘可能显示出组织学变化，这些变化可能与病毒直接相关，也可能无关。对早期妊娠胎盘组织的分析可能在阐明母婴HIV传播机制方面提供更多信息。
