Tugcu Volkan, Kemahli Eray, Ozbek Emin, Arinci Yasar Volkan, Uhri Mehmet, Erturkuner Pelin, Metin Gokhan, Seckin Ismail, Karaca Cetin, Ipekoglu Nursen, Altug Tuncay, Cekmen Mustafa Baki, Tasci Ali Ihsan
Department of Urology, Bakirkoy Research and Training Hospital, Istanbul, Turkey.
J Endourol. 2008 Dec;22(12):2723-31. doi: 10.1089/end.2008.0357.
We aimed to study the protective effects of pomegranate juice (PJ) on ethylene glycol (EG)-induced crystal deposition in renal tubules, renal toxicity, and inducible nitric oxide synthase (iNOS) and nuclear factor-kappaB activities in rat kidneys.
Fifty-six rats were divided into four equal groups: Control, EG, EG + 50 microL PJ/d (PJ50), and EG + 100 microL PJ/d (PJ100). Rats were sacrified on days 10 and 45. Tissue sections were evaluated under light and polarized microscopy for the presence and degree of crystal deposition and toxicity in the kidneys. Crude extracts of the cortex were used to determine reduced gluthatione (GSH), nitric oxide (NO), and malondialdehyde (MDA) levels.
In the EG group, crystal depositions were more evident and mild crystalization was observed in proximal tubules on day 10; severe crystalization and granulovacuolar epithelial cell degeneration were observed on day 45. There was limited or no crystal formation in the EG + PJ-given groups. There were completely normal renal and tubular structures in the control group. There was no significant difference between the four groups in serum levels of sodium, potassium, blood urea nitrogen, and creatinine in any sampling time. Hyperoxaluria, a marked increase in MDA and NO levels, and decrease of GSH were observed in the EG-given groups compared with the others. There were marked iNOS and p65 expressions in only the EG-given rats compared with control and PJ groups, immunohistochemically.
This experiment shows the protective effect of PJ in the EG-induced crystal depositions in renal tubules.
我们旨在研究石榴汁（PJ）对乙二醇（EG）诱导的大鼠肾小管晶体沉积、肾毒性以及诱导型一氧化氮合酶（iNOS）和核因子κB活性的保护作用。
56只大鼠被分为四组，每组数量相等：对照组、EG组、EG + 50 μL PJ/天（PJ50组）和EG + 100 μL PJ/天（PJ100组）。在第10天和第45天处死大鼠。对组织切片进行光镜和偏光显微镜检查，以评估肾脏中晶体沉积的存在情况和程度以及毒性。用皮质粗提物测定还原型谷胱甘肽（GSH）、一氧化氮（NO）和丙二醛（MDA）水平。
在EG组中，第10天时晶体沉积更明显，近端小管出现轻度结晶；第45天时观察到严重结晶和颗粒空泡状上皮细胞变性。在给予EG + PJ的组中晶体形成有限或未形成。对照组肾脏和肾小管结构完全正常。在任何采样时间，四组之间血清钠、钾、血尿素氮和肌酐水平均无显著差异。与其他组相比，给予EG的组出现高草酸尿症、MDA和NO水平显著升高以及GSH降低。免疫组化结果显示，与对照组和PJ组相比，仅给予EG的大鼠中有明显的iNOS和p65表达。
本实验表明PJ对EG诱导的肾小管晶体沉积具有保护作用。
