Guay Claudiane, Goudreault Danielle, Schänzer Wilhem, Flenker Ulrich, Ayotte Christiane
Laboratoire de contrôle du dopage, INRS-Institut Armand-Frappier, Laval, Québec, Canada.
Steroids. 2009 Mar;74(3):350-8. doi: 10.1016/j.steroids.2008.10.015. Epub 2008 Nov 5.
The urinary phase II metabolites of norsteroids, 19-norandrosterone, 19-noretiocholanolone and 19-norepiandrosterone glucuronide and sulphate, were analyzed in samples collected during the pregnancy, following the administration of norsteroids or the consumption of edible parts of non-castrated pig and in athletes' samples in which they were found during routine controls. The level of the sulfo- and glucuroconjugated metabolites was precisely determined by GC/HRMS, after selective hydrolysis. The goal was to evaluate whether the fine analysis of the norsteroid conjugates produced and excreted in different conditions would show a pattern that could be linked to their origin. The delta (13)C values of the metabolites formed following the ingestion of edible parts of non-castrated pig were measured by isotope ratio mass spectrometry. Our results indicated that it is not possible to determine the origin of the urinary metabolites based upon the sole evaluation of the different metabolites and conjugates. The GC/C/IRMS is the only method permitting to distinguish between the exogenous and endogenous origin of the metabolites.
在孕期收集的样本中，对去甲类固醇、19-去氢表雄酮、19-去甲本胆烷醇酮以及19-去甲表雄酮葡糖醛酸苷和硫酸盐的尿二期代谢产物进行了分析，这些样本来自于给予去甲类固醇后、食用未阉割猪的可食用部分后，以及在运动员样本中（在常规检测中发现了这些代谢产物）。在选择性水解后，通过气相色谱/高分辨质谱法精确测定了磺酸和葡糖醛酸结合代谢产物的水平。目的是评估对在不同条件下产生和排泄的去甲类固醇结合物进行精细分析是否会显示出一种与它们的来源相关的模式。通过同位素比率质谱法测量了摄入未阉割猪的可食用部分后形成的代谢产物的δ(13)C值。我们的结果表明，仅基于对不同代谢产物和结合物的评估，无法确定尿代谢产物的来源。气相色谱/燃烧/同位素比率质谱法是唯一能够区分代谢产物外源性和内源性来源的方法。
