Wang Shu-Bin, Chen Shu-Ping, Gao Yong-Hui, Luo Ming-Fu, Liu Jun-Ling
Department of Physiology, Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, 16 Nanxiaojie, Dongzhimennei, Beijing 100700, China.
World J Gastroenterol. 2008 Nov 14;14(42):6496-502. doi: 10.3748/wjg.14.6496.
To observe the effect of electroacupuncture (EA) of "Neiguan" (PC6) and "Gongsun" (SP4) on pathological changes of the heart and stomach in rats with acute myocardial ischemia (AMI), and to explore its underlying mechanism.
Fifty Wistar rats were randomized into control, model, PC6, SP4 and PC6 + SP4 groups (n = 8 each group). An AMI model was established by occlusion of the descending anterior branch (DAB) of the left coronary artery. ECG-ST of cervico-thoracic lead and electrogastrogram (EGG) were recorded. EA was applied to PC6, SP4 and PC6 + SP4 groups, respectively. At the end of experiments, the rats were transcardically perfused with 4% paraformaldehyde, and the heart base myocardium, gastric antrum and duodenum tissues were sampled, sectioned and stained with a reduced form of nicotinamide-adenine dinucleotide phosphate (NADPH)-diaphorase histochemical method for displaying nitric oxide synthase (NOS) activity.
After AMI, ECG-ST values elevated. After EA, the elevated ECG-ST values at 20 min in PC6 group, at 30 min in PC6 + SP4 and SP4 groups had no significant differences in comparison with their respective basal values before AMI. Following AMI, the amplitude and frequency of slow waves of EGG decreased remarkably (P < 0.05). At 30 min after EA, the mean amplitude and frequency of slow waves of EGG in the three EA groups had no marked differences compared with their individual basal levels and those in the control group. After AMI, the mean integral grey values of NOS-positive product in myocardium, gastric antrum and duodenum tissues in the model group increased remarkably in comparison with the control group, while those in three EA groups were lower than those in the model group. No significant differences were found in ECG-ST and EGG improvement among the three EA groups. However, EA of PC6 had a better effect on ECG-ST and EA of PC4 had a better effect on EGG, respectively.
EA of PC6, SP4 and PC6 + SP4 can significantly promote the recovery of cardiac and gastric electrical activities after AMI, and up-regulate NOS expression in myocardium, gastric antrum and duodenum tissues.
观察电针“内关”（PC6）和“公孙”（SP4）对急性心肌缺血（AMI）大鼠心脏和胃病理变化的影响，并探讨其潜在机制。
将50只Wistar大鼠随机分为对照组、模型组、PC6组、SP4组和PC6 + SP4组（每组n = 8）。通过结扎左冠状动脉前降支建立AMI模型。记录颈胸导联心电图ST段及胃电图（EGG）。分别对PC6组、SP4组和PC6 + SP4组进行电针治疗。实验结束时，经心内灌注4%多聚甲醛，取心脏基部心肌、胃窦和十二指肠组织，切片，采用还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）-黄递酶组织化学方法染色，以显示一氧化氮合酶（NOS）活性。
AMI后，心电图ST段值升高。电针后，PC6组在20分钟时、PC6 + SP4组和SP4组在30分钟时升高的心电图ST段值与AMI前各自的基础值相比无显著差异。AMI后，EGG慢波的振幅和频率显著降低（P < 0.05）。电针后30分钟，三个电针组EGG慢波的平均振幅和频率与各自的基础水平及对照组相比无明显差异。AMI后，模型组心肌、胃窦和十二指肠组织中NOS阳性产物的平均积分灰度值与对照组相比显著增加，而三个电针组低于模型组。三个电针组在心电图ST段和EGG改善方面无显著差异。然而，PC6电针对心电图ST段有较好的影响，PC4电针对EGG有较好的影响。
PC6、SP4及PC6 + SP4电针可显著促进AMI后心脏和胃电活动的恢复，并上调心肌、胃窦和十二指肠组织中NOS的表达。
