[湖南低龄组乙型肝炎病毒基因特征及突变率分析]

[The analysis of hepatitis B virus genetic characterization and ratio of mutation in low-age group of HuNan].

作者信息

Bian Tao, Zhang Yong, Cao Yan-Qiang, Wang Ji-Jie, Shen Li-Ping, Wang Feng, Wang Yue, Bi Sheng-Li

机构信息

Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008 Jun;22(3):205-7.

PMID:19031704

Abstract

OBJECTIVE

In order to investigate the characterization of mutation and genotype distributing in the younger group which was under the universal vaccination. The sequence of HBV was analyzed to offer the information to control and prevention in the area.

METHODS

Young person's sera with positive HBsAg are collected, and the Large S sequence of HBV including preS and S gene are amplified and sequenced. The genotype and serotype were determined by clastwal with the standard genotype sequence. And one virus complete genome is amplified.

RESULTS

The virus gene are successful amplified from the 33 sera. The sequence result indicate the 30 of 33 (90.9%) HBV genotype is B and 3 of 33 (9.0%) is C. The HBV serotype including ayw (1), adr (3), adw (29), 5 of 33 mutated in the "a" dominant of HBV, and the percentage is 15.2% . The HBV full length gene of serum number of 5856 is amplified and sequenced. Its genotype is B, serotype is adw and length is 3215 base.

CONCLUSIONS

The dominant genotype of HuNan is B, and the dominant serotype is adw.

摘要

目的

为研究在普遍接种疫苗情况下年轻人群中乙型肝炎病毒（HBV）的突变特征及基因型分布情况。通过分析HBV序列，为该地区的防控工作提供信息。

方法

收集HBsAg阳性的年轻人血清，扩增并测序HBV的大S序列（包括前S和S基因）。采用与标准基因型序列比对的方法确定基因型和血清型。并扩增一个病毒全基因组。

结果

成功从33份血清中扩增出病毒基因。序列结果显示，33株HBV中30株（90.9%）基因型为B型，3株（9.0%）为C型。HBV血清型包括ayw（1株）、adr（3株）、adw（29株），33株中有5株在HBV “a” 决定簇处发生突变，占比15.2%。扩增并测序了5856号血清的HBV全长基因，其基因型为B型，血清型为adw型，长度为3215个碱基。