[青海同德藏族人群乙型肝炎病毒基因型及血清型分布调查]
[Investigation on distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghai].
作者信息
Zang Jie, Jiang Shuang-Ying, Wang Xue-Wen, Wang Feng, Bian Tao, Mu Zhen-Yun, Zhang Yong
机构信息
Department of Epidemiology and Healthy Statistics, Hebei Medical University, Shijiazhuang 050017, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Feb;23(1):2-4.
OBJECTIVE
To explore the distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghai.
METHODS
Nested polymerase chain reaction (nPCR) was used for amplification of S gene and C gene of HBV from sera carried by Tibetan chronic HBV carrier in Tongde, Qinghai, then the HBV DNA positive products were sequenced by direct sequencing. Genotype and serotype were identified by analysis of sequence result.
RESULTS
271, which come from 311 sera samples with positive HBsAg randomly selected from natural community, were amplified and sequenced in both S gene and C gene successfully, 10 (3.7%), 261 (96.3%) out of them were identified as genotype C, recombinant between genotypes C and D respectively; 259 (95.6%), 10 (3.7%), 2 (0.7%) belonged to serotype ayw2, adr, adw2 respectively.
CONCLUSION
The recombinant between genotypes C and D was the main genotype in Tibetan chronic carrier with hepatitis Bin Tongde, Qinghai; the serotype of this areas was consisted largely of ayw2.
目的
探讨青海同德藏族人群乙型肝炎病毒（HBV）基因型及血清型分布情况。
方法
采用巢式聚合酶链反应（nPCR）对青海同德藏族慢性HBV携带者血清中的HBV S基因和C基因进行扩增，对HBV DNA阳性产物进行直接测序，通过序列分析确定基因型及血清型。
结果
从自然人群中随机抽取311份HBsAg阳性血清标本，成功对其中271份进行了S基因和C基因的扩增及测序，其中10份（3.7%）、261份（96.3%）分别被鉴定为C基因型、C和D基因型重组型；血清型分别为ayw2型259份（95.6%）、adr型10份（3.7%）、adw2型2份（0.7%）。
结论
青海同德藏族慢性乙肝携带者中C和D基因型重组型为主要基因型；该地区血清型以ayw2为主。
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