Cheng De-Xi, Ye Guo-Liang, Lin Ya, Chen Zhi-Hui
Xiang Shan County First People Hospital, Zhejiang 315700, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Jun;23(3):194-6.
In order to investigate the characterization of mother to children transmission, the sequences of HBV were analyzed to offer the information about the effect of interrupt.
The sera of 75 mother with positive HBsAg are collected from 2003, and the ELISA was performed to determine the HBV infection of the child. The Large S sequence of HBV including preS and S gene are amplified and sequenced. The genotype was determined with the standard genotype sequence. The mutation ratios of group successfully interrupted and failed compared.
The sera of 4 pairs mother-children were HBsAg positive, including one twins. The virus genes are successful amplified. Four of HBV genotype is B and one is C. Gene of twins has mutation of T143M. 43 HBV of successfully interrupted group were sequenced. There are 37 of genotype B and 6 of genotype C. Three have the mutation in "a" dominant, and the percentage is 7%.
Most failed interrupted child have the same sequence with their mother, and the ratio is higher than the mother of successful group, however there have no statistical significance.
为研究乙肝病毒母婴传播的特征，对乙肝病毒序列进行分析，以提供关于阻断效果的信息。
收集2003年以来75例乙肝表面抗原阳性母亲的血清，采用酶联免疫吸附试验检测其子女的乙肝病毒感染情况。对乙肝病毒大S序列（包括前S和S基因）进行扩增和测序。根据标准基因型序列确定基因型。比较成功阻断组和失败组的突变率。
4对母婴血清乙肝表面抗原阳性，其中1对为双胞胎。病毒基因成功扩增。4例乙肝病毒基因型为B型，1例为C型。双胞胎的基因有T143M突变。对成功阻断组的43例乙肝病毒进行测序。其中37例为B型基因型，6例为C型基因型。3例在“a”决定簇有突变，占7%。
大多数阻断失败的儿童与母亲序列相同，其比例高于成功组母亲，但无统计学意义。
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