Takahashi O
Department of Toxicology, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
Food Chem Toxicol. 1991 Mar;29(3):173-83. doi: 10.1016/0278-6915(91)90035-6.
The platelet aggregation characteristics of male Sprague-Dawley (Jcl:SD) rats were investigated. Epinephrine, ristocetin, serotonin and platelet-activating factor were ineffective in rat platelets. Heparinized platelet-rich plasma (PRP) was more sensitive than citrated PRP to three aggregating agents, ADP, collagen and arachidonic acid. Butylated hydroxytoluene (BHT) and BHT quinone methide (2,6-di-tert-butyl-4-methylene-2,5-cyclohexadienone) inhibited ADP- and collagen-induced aggregation at concentrations over 10(-3) M in vitro. The ADP-, collagen- and arachidonic acid (0.5-2.0 mM)-induced aggregations of PRP obtained from rats given 1.20% BHT in the diet for 7 days were normal, while arachidonic acid (3.9 mM)-induced aggregation of PRP from BHT-fed rats was significantly lower than control. PRP from rats given aspirin and warfarin also aggregated normally with ADP or collagen addition. These results suggest that heparinized PRP may be preferable in platelet aggregation analyses in rats and reaffirmed that effects on platelet aggregation may not play a key role in BHT-induced bleeding. Platelet aggregation capacity also does not necessarily reduce in haemorrhages induced by aspirin or warfarin.
对雄性斯普拉格-道利（Jcl:SD）大鼠的血小板聚集特性进行了研究。肾上腺素、瑞斯托霉素、血清素和血小板活化因子对大鼠血小板无效。肝素化富血小板血浆（PRP）比枸橼酸化PRP对三种聚集剂（ADP、胶原和花生四烯酸）更敏感。丁基羟基甲苯（BHT）和丁基羟基甲苯醌甲基化物（2,6-二叔丁基-4-亚甲基-2,5-环己二烯酮）在体外浓度超过10^(-3) M时可抑制ADP和胶原诱导的聚集。给大鼠喂食含1.20% BHT的饲料7天，所获得的PRP对ADP、胶原和花生四烯酸（0.5 - 2.0 mM）诱导的聚集正常，而BHT喂养大鼠的PRP对花生四烯酸（3.9 mM）诱导的聚集明显低于对照组。给予阿司匹林和华法林的大鼠的PRP在添加ADP或胶原时也能正常聚集。这些结果表明，在大鼠血小板聚集分析中，肝素化PRP可能更可取，并重申对血小板聚集的影响可能在BHT诱导的出血中不发挥关键作用。在阿司匹林或华法林诱导的出血中，血小板聚集能力也不一定降低。
