用于检测从鳟鱼饲料中分离出的黄曲霉菌株产黄曲霉毒素的不同培养基和方法。

Different media and methodologies for the detection of aflatoxin production by Aspergillus flavus strains isolated from trout feed.

作者信息

Cutuli M T, Cuellar A, Camara J M, Mateos A, Suarez G

机构信息

U.D. Microbiologia e Inmunologia, Dpto. Patologia Animal, Facultad de Veterinaria, Universidad, Complutense, Madrid, Spain.

出版信息

Mycopathologia. 1991 Feb;113(2):121-5. doi: 10.1007/BF00442423.

DOI:10.1007/BF00442423

PMID:1903512

Abstract

We have studied the aflatoxin producing capacity of 41 Aspergillus flavus strains isolated from the mycoflora present of natural media (wheat, rice and mixed feed) synthetic medium (Aflatoxin Producing Ability Medium) and semisynthetic media (Coconut Agar Medium and Glucose Yeast Extract Agar) were compared. Aflatoxins were analysed on days 4 and 8 post-inoculation under an incubation temperature of 28 degrees C. A total of 30 strains (75.7%) were producers on natural media as detected by Thin Layer Chromatography: 23 strains on wheat, 27 on rice and 12 on mixed feed. The results by qualitative fluorescence tests on synthetic and semisynthetic media were: 3 strains positive on Coconut Agar Medium (CAM) 1 on Glucose Yeast Extract Agar (GY + Agar) and none on Aflatoxin Producing Ability Medium (APA).

摘要

我们研究了从天然培养基（小麦、大米和混合饲料）中存在的霉菌菌群分离出的41株黄曲霉产生黄曲霉毒素的能力，并比较了合成培养基（黄曲霉毒素产生能力培养基）和半合成培养基（椰子琼脂培养基和葡萄糖酵母提取物琼脂）。在28℃的培养温度下，于接种后第4天和第8天分析黄曲霉毒素。通过薄层色谱法检测，共有30株（75.7%）菌株在天然培养基上产生黄曲霉毒素：23株在小麦上，27株在大米上，12株在混合饲料上。在合成和半合成培养基上进行定性荧光试验的结果如下：3株在椰子琼脂培养基（CAM）上呈阳性，1株在葡萄糖酵母提取物琼脂（GY+琼脂）上呈阳性，在黄曲霉毒素产生能力培养基（APA）上均无阳性。