Jiang Wen, Song Lei, Shao Jian-Guo, Wang Lu-Jun, Lu Jian-Rong, Liu Hai-Ou
Department of Gastroenterology, Nantong Third People's Hospital, Nantong 226006, China.
Zhonghua Nei Ke Za Zhi. 2008 Jul;47(7):570-3.
To investigate the change and effect of SSeCKS (src suppressed c kinase substrates) in the activation of hepatic stellate cells (HSCs).
HSCs were isolated from normal rats, the change of SSeCKS mRNA expression on HSCs culture in vitro was determined using real-time PCR, protein level was determined by Western blot and immunofluorescence methods. A rat model of liver fibrosis was established. The expression and location of SSeCKS and alpha-SMA (alpha-smooth muscle actin) in liver tissues were detected by immunofluorescence methods.
SSeCKS mRNA expression was low in freshly isolated HSCs cell and the expression increased in activated HSCs in vitro. In liver fibrosis tissue, the number of SSeCKS-positive cells was increased and these cells were distributed along the sinusoids which also contained alpha-SMA positive cells.
The expression of SSeCKS was increased in activated HSCs in vitro. Therefore, SSeCKS may be involved in the liver inflammation and fibrosis.
研究富含半胱氨酸的酸性分泌蛋白（SSeCKS，src抑制的c激酶底物）在肝星状细胞（HSCs）激活过程中的变化及作用。
从正常大鼠中分离出肝星状细胞，采用实时定量聚合酶链反应（real-time PCR）检测体外培养的肝星状细胞中SSeCKS mRNA表达的变化，通过蛋白质免疫印迹法和免疫荧光法测定蛋白质水平。建立大鼠肝纤维化模型，采用免疫荧光法检测肝组织中SSeCKS和α-平滑肌肌动蛋白（α-SMA）的表达及定位。
新鲜分离的肝星状细胞中SSeCKS mRNA表达较低，体外激活的肝星状细胞中表达增加。在肝纤维化组织中，SSeCKS阳性细胞数量增加，这些细胞沿含有α-SMA阳性细胞的肝血窦分布。
体外激活的肝星状细胞中SSeCKS表达增加。因此，SSeCKS可能参与肝脏炎症和纤维化过程。
